Abstract breath test2.docx
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Abstract breath test2.docx
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Abstractbreathtest2
Abstractbreathtest2
AnalChem. 2015Jan20;87
(2):
982-8.doi:
10.1021/ac503450y.Epub2014Dec29.
Fastandhighlysensitivefiber-enhancedRamanspectroscopicmonitoringofmolecularH2andCH4forpoint-of-carediagnosisofmalabsorptiondisordersinexhaledhuman breath.
HanfS1, BögöziT, KeinerR, FroschT, PoppJ.
Authorinformation
∙1LeibnizInstituteofPhotonicTechnology,Jena07745,Germany.
Abstract
Breath gasanalysisisanovelpowerfultechniquefornoninvasive,early-stagediagnosisofmetabolicdisordersordiseases.Molecularhydrogenand methane arebiomarkersforcolonicfermentation,becauseofmalabsorptionofoligosaccharides(e.g.,lactoseorfructose)andforsmallintestinalbacterialovergrowth.Recently,thepresenceofthesegasesinexhaled breath wasalsocorrelatedwithobesity.Here,wereportonthehighlyselectiveandsensitivedetectionofmolecularhydrogenand methane withinacomplexgasmixture(consistingofH2,CH4,N2,O2,andCO2)bymeansoffiber-enhancedRamanspectroscopy(FERS).AnelaborateFERSsetupwithamicrostructuredhollowcorephotoniccrystalfiber(HCPCF)providedahighlyimprovedanalyticalsensitivity.ThesimultaneousmonitoringofH2withallothergaseswasachievedbyacombinationofrotational(H2)andvibrational(othergases)RamanspectroscopywithinthelimitedspectraltransmissionrangeoftheHCPCF.TheHCPCFwascombinedwithanadjustableimage-planeaperturepinhole,inordertoseparatetheH2rotationalRamanbandsfromthesilicabackgroundsignalandimprovethesensitivitydowntoalimitofdetection(LOD)of4.7ppm(foronly26fmolH2).TheabilitytomonitorthelevelsofH2andCH4inapositivehydrogen breath test(HBT)wasdemonstrated.TheFERSsensorpossessesahighdynamicrange(∼5ordersofmagnitude)withafastresponsetimeoffewsecondsandprovidesgreatpotentialforminiaturization.Weforeseethatthistechniquewillpavethewayforfast,noninvasive,andpainlesspoint-of-carediagnosisofmetabolicdiseasesinexhaledhuman breath.
Reviewarticle:
breath analysisininflammatoryboweldiseases.
KuradaS1, AlkhouriN, FiocchiC, DweikR, RiederF.
Authorinformation
∙1DepartmentofHospitalMedicine,MedicineInstitute,Cleveland,OH,USA.
Abstract
BACKGROUND:
Thereisanurgentneedforcheap,reproducible,easytoperformandspecificbiomarkersfordiagnosis,differentiationandstratificationofinflammatoryboweldisease(IBD)patients.Technicaladvancesallowforthedeterminationofvolatileorganiccompoundsinthehuman breath todifferentiatebetweenhealthanddisease.
AIM:
Reviewanddiscussmedicalliteratureonvolatileorganiccompoundsinexhaledhuman breath inGIdisorders,focusingondiagnosisanddifferentiationofIBD.
METHODS:
AsystematicsearchinPubMed,OvidMedlineandScopuswascompletedusingappropriatekeywords.Inaddition,abibliographysearchofeacharticlewasperformed.
RESULTS:
Mean breath pentane,ethane,propane,1-octene,3-methylhexane,1-deceneandNOlevelswereelevated(P<0.05toP<10(-7))andmean breath 1-nonene,(E)-2-nonene,hydrogensulphideand methane weredecreasedinIBDcomparedtohealthycontrols(P=0.003toP<0.001).Acombinedpanelof3volatileorganiccompounds(octene,(E)-2-noneneanddecene)showedthebestdiscriminationbetweenpaediatricIBDandcontrols(AUC0.96). Breath condensatecytokineswerehigherinIBDcomparedtohealthyindividuals(P<0.008). Breath pentane,ethane,propane,isopreneandNOlevelscorrelatedwithdiseaseactivityinIBDpatients. Breath condensateinterleukin-1βshowedaninverserelationwithclinicaldiseaseactivity.
CONCLUSIONS:
Breath analysisinIBDisapromisingapproachthatisnotyetreadyforroutineclinicaluse,butdatafromothergastrointestinaldiseasessuggestthefeasibilityforuseofthistechnologyinclinicalpractice.Well-designedfuturetrials,incorporatingthelatest breath detectiontechniques,needtodeterminetheexact breath metabolomepatternlinkedtodiagnosisandphenotypeofIBD.
WorldJGastroenterol. 2014Nov21;20(43):
16062-78.doi:
10.3748/wjg.v20.i43.16062.
Archaeaandthehumangut:
newbeginningofanoldstory.
GaciN1, BorrelG1, TotteyW1, O'ToolePW1, BrugèreJF1.
Authorinformation
∙1NadiaGaci,WilliamTottey,Jean-FrançoisBrugère,EA-4678CIDAM,ClermontUniversité,Universitéd'Auvergne,F-63000Clermont-Ferrand,France.
Abstract
Methanogenicarchaeaareknownashumangutinhabitantssincemorethan30yearsagothroughthedetectionof methane inthe breath andisolationoftwomethanogenicspeciesbelongingtotheorderMethanobacteriales,MethanobrevibactersmithiiandMethanosphaerastadtmanae.Duringthelastdecade,diversityofarchaeaencounteredinthehumangastrointestinaltract(GIT)hasbeenextendedbysequenceidentificationandculturingofnewstrains.HereweprovideanupdatedcensusofthearchaealdiversityassociatedwiththehumanGITandtheirpossibleroleinthegutphysiologyandhealth.Weparticularlyfocusonthestillpoorlycharacterized7thorderofmethanogens,theMethanomassiliicoccales,associatedtoagedpopulation.Whilealsolargelydistributedinnon-GITenvironments,ouractualknowledgeonthisnovelorderofmethanogenshasbeenmainlyrevealedthroughGITinhabitants.Theyenlargethenumberoffinalelectronacceptorsofthegutmetabolitestomono-di-andtrimethylamine.Trimethylamineisexclusivelyamicrobiota-derivedproductofnutrients(lecithin,choline,TMAO,L-carnitine)fromnormaldiet,fromwhichseemsoriginatetwodiseases,trimethylaminuria(orFish-OdorSyndrome)andcardiovasculardiseasethroughtheproatherogenicpropertyofitsoxidizedliver-derivedform.Thisthereforesupportsinterestonthesemethanogenicspeciesanditsuseasarchaebiotics,atermcoinedfromthenotionofarchaea-derivedprobiotics.
GutMicrobes. 2014Mar-Apr;5
(2):
165-75.doi:
10.4161/gmic.27923.Epub2014Jan27.
Gutmicrobiotainfluenceslowfermentablesubstratedietefficacyinchildrenwithirritablebowelsyndrome.
ChumpitaziBP1, HollisterEB2, OezguenN2, TsaiCM1, McMeansAR3, LunaRA2, SavidgeTC2, VersalovicJ4, ShulmanRJ5.
Authorinformation
∙1DepartmentofPediatrics;BaylorCollegeofMedicine;Houston,TXUSA;SectionofPediatricGastroenterology,Hepatology,andNutrition;TexasChildren'sHospital;Houston,TXUSA.
∙2DepartmentofPathologyandImmunology;BaylorCollegeofMedicine;Houston,TXUSA;TexasChildren'sMicrobiomeCenter;DepartmentofPathology;TexasChildren'sHospital;Houston,TXUSA.
∙3Children'sNutritionResearchCenter;Houston,TXUSA.
∙4DepartmentofPediatrics;BaylorCollegeofMedicine;Houston,TXUSA;DepartmentofPathologyandImmunology;BaylorCollegeofMedicine;Houston,TXUSA;TexasChildren'sMicrobiomeCenter;DepartmentofPathology;TexasChildren'sHospital;Houston,TXUSA.
∙5DepartmentofPediatrics;BaylorCollegeofMedicine;Houston,TXUSA;SectionofPediatricGastroenterology,Hepatology,andNutrition;TexasChildren'sHospital;Houston,TXUSA;Children'sNutritionResearchCenter;Houston,TXUSA.
Abstract
Wesoughttodeterminewhetheralowfermentablesubstratediet(LFSD)decreasesabdominalpainfrequencyinchildrenwithirritablebowelsyndrome(IBS)andtoidentifypotentialmicrobialfactorsrelatedtodietefficacy.Painsymptoms,stoolingcharacteristics, breath hydrogenandmethane,wholeintestinaltransittime,stoolmicrobiome,andmetabolitecompositionwerecollectedand/ordocumentedineightchildrenwithIBSatbaselineandduringoneweekofanLFSDintervention.Painfrequency(P<0.05),painseverity(P<0.05),andpain-relatedinterferencewithactivities(P<0.05)decreasedinthesubjectswhileontheLFSD.Respondersvs.non-responders:
fourchildren(50%)wereidentifiedasresponders(>50%decreaseinabdominalpainfrequencywhileontheLFSD).Therewerenodifferencesbetweenrespondersandnon-responderswithrespecttohydrogenproduction, methane production,stoolingcharacteristics,orguttransittime.Responderswerecharacterizedbyincreasedpre-LFSDabundanceofbacterialtaxabelongingtothegeneraSporobacter(P<0.05)andSubdoligranulum(P<0.02)anddecreasedabundanceoftaxabelongingtoBacteroides(P<0.05)relativetonon-responders.Inparallel,stoolmetabolitesdifferedbetweenrespondersandnon-respondersandwereassociatedwithdifferencesinmicrobiomecomposition.ThesepilotstudyresultssuggestthatanLFSDmaybeeffectiveindecreasingGIsymptomsinchildrenwithIBS.MicrobialfactorssuchasgutmicrobiomecompositionandstoolmetaboliteswhileonthedietmayrelatetoLFSDefficacy.
UnitedEuropeanGastroenterolJ. 2014Apr;2
(2):
131-7.doi:
10.1177/2050640614521124.
Symptomaticfructosemalabsorptioninirritablebowelsyndrome:
Aprospectivestudy.
MelchiorC1, GourcerolG2, DéchelotteP3, LeroiAM2, DucrottéP1.
Authorinformation
∙1GastroenterologyDepartment,RouenUniversityHospital,Rouen,France;INSERMUMR-1073,RouenUniversityHospital,Rouen,France.
∙2INSERMUMR-1073,RouenUniversityHospital,Rouen,France;PhysiologyDepartment,RouenUniversityHospital,Rouen,France.
∙3INSERMUMR-1073,RouenUniversityHospital,Rouen,France;NutritionUnit,RouenUniversityHospital,Rouen,France.
Abstract
INTRODUCTION:
Fructosecantriggerorworsensymptomsinirritablebowelsyndrome(IBS)patients.TheaimofthisstudywastodeterminetheprevalenceofsymptomaticfructosemalabsorptioninIBSpatientsandtotestwhetherthepatient'scharacteristicscanhelptodetectafructosemalabsorption.
MATERIALSANDMETHODS:
NinetyRomeIIIIBSpatients(predominantdiarrhoea(IBS-D):
31%,predominantconstipation(IBS-C):
18%,mixedtype(IBS-M):
51%)wereinclud
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