各种缓冲溶液配制表-.docx
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各种缓冲溶液配制表-.docx
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各种缓冲溶液配制表-
实验室常用缓冲液的配制方法
1.甘氨酸-盐酸缓冲液(0.05M)
Xml0.2M甘氨酸+Yml0.2M盐酸,再加水稀释至200ml。
pHX/mlY/mlpHX/mlY/ml
2.25044.03.05011.4
2.45032.43.2508.2
2.65024.23.4506.4
2.85016.83.6505.0
甘氨酸分子量=75.070.2M甘氨酸溶液含15.01g/L
2.邻苯二甲酸-盐酸缓冲液(0.05M)
Xml0.2M邻苯二甲酸氢钾+Yml0.2M盐酸,再加水稀释至200ml。
pHX/mlY/mlpHX/mlY/ml
2.254.6703.251.470
2.453.9603.450.990
2.653.2953.650.597
2.852.6423.850.263
3.052.032
邻苯二甲酸氢钾分子量=204.230.2M邻苯二甲酸氢钾溶液含40.85g/L
3.磷酸氢二钠-柠檬酸缓冲液
pH0.2MNa2HPO4/ml0.1M柠檬酸/mlpH0.2MNa2HPO4/ml0.1M柠檬酸/ml
2.20.4019.65.210.729.28
2.41.2418.765.411.158.85
2.62.1817.825.611.608.40
2.83.1716.835.812.097.91
3.04.1115.896.012.637.37
3.24.9415.066.213.226.78
3.45.7014.306.413.856.15
3.66.4413.566.614.555.45
3.87.1012.906.815.454.55
4.07.7112.297.016.473.53
4.28.2811.727.217.392.61
4.48.8211.187.418.171.83
4.69.3510.657.618.731.27
4.89.8610.147.819.150.85
5.010.309.708.019.450.55
Na2HPO4分子量=41.980.2M溶液含28.40g/L
Na2HPO4·2H2O分子量=78.050.2M溶液含35.61g
C6H8O7·H2O分子量=10.140.1M溶液含21.01g/L
4.柠檬酸-氢氧化钠-盐酸缓冲液
pH钠离子浓度/M柠檬酸C6H8O7·H2O/g氢氧化钠NaOH/g浓盐酸HCl/ml终体积/L
2.20.202108416010
3.10.202108311610
3.30.202108310610
4.30.20210834510
5.30.352451446810
5.80.4528518610510
6.50.3826615612610
使用时可以每升中加入1g酚,若最后pH有变化,再用少量50%氢氧化钠溶液或浓盐酸调节,冰箱保存。
柠檬酸-柠檬酸钠缓冲液(0.1M)
pH0.1M柠檬酸/ml0.1M柠檬酸钠/mlpH0.1M柠檬酸/ml0.1M柠檬酸钠/ml
3.018.61.45.08.211.8
3.217.22.85.27.312.7
3.416.04.05.46.413.6
3.614.95.15.65.514.5
3.814.06.05.84.715.3
4.013.16.96.03.816.2
4.212.37.76.22.817.2
4.411.48.66.42.018
4.610.39.76.61.418.6
4.89.210.8
C6H8O7·H2O分子量=10.140.1M溶液含21.01g/L
Na3C6H5O7·2H2O分子量=294.120.1M溶液含29.41g/L
5.乙酸-乙酸钠缓冲液(0.2M)
pH0.2MNaAc/ml0.2MHAC/mlpH0.2MNaAc/ml0.2MHAC/ml
3.60.759.254.85.904.10
3.81.208.805.07.003.00
4.01.808.205.27.902.10
4.22.657.355.48.601.40
4.43.706.305.69.100.90
4.64.905.105.89.400.60
NaAc分子量=136.090.2M溶液为27.22g/L
6.磷酸盐缓冲液
(1)磷酸氢二钠-磷酸二氢钠缓冲液(0.2M)详见常用磷酸缓冲液的配制方法
(2)磷酸氢二钠-磷酸二氢钾缓冲液(1/15M=cM)
pHcMNaH2PO4/mlcMKH2PO4/mlpHcMNaH2PO4/mlcMKH2PO4/ml
4.920.109.907.177.003.00
5.290.509.507.388.002.00
5.911.009.007.739.001.00
6.242.008.008.049.500.50
6.473.007.008.349.750.25
6.644.006.008.679.900.10
6.815.005.009.1810.000
6.986.004.00
Na2HPO4·2H2O分子量=178.05
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