Crude Polysaccharides Extracted From Several Medicinal Fungi Exhibit Hypoglycemic Functions in Mice.docx
- 文档编号:7225816
- 上传时间:2023-01-22
- 格式:DOCX
- 页数:5
- 大小:18.80KB
Crude Polysaccharides Extracted From Several Medicinal Fungi Exhibit Hypoglycemic Functions in Mice.docx
《Crude Polysaccharides Extracted From Several Medicinal Fungi Exhibit Hypoglycemic Functions in Mice.docx》由会员分享,可在线阅读,更多相关《Crude Polysaccharides Extracted From Several Medicinal Fungi Exhibit Hypoglycemic Functions in Mice.docx(5页珍藏版)》请在冰豆网上搜索。
CrudePolysaccharidesExtractedFromSeveralMedicinalFungiExhibitHypoglycemicFunctionsinMice
CrudePolysaccharidesExtractedFromSeveralMedicinalFungiExhibitHypoglycemicFunctionsinMice
Abstract:
Thehypoglycemiceffectsofcrudepolysaccharidesextractedfromfivemedicinalfungiwereevaluatedusingalloxan-induceddiabeticmice.High(1g/kg)andlow(0.5g/kg)dosesofpolysaccharidesextractedfromCordycepstaiiandC.memorabiliscausedsignificant(P<0.01)decreasesinmicebloodsugarlevels.PolysaccharidesfromGanodermalucidumandC.militarisfruitbodiesbothexhibitedweakhypoglycemiceffectsatsignificancelevelsofP<0.01andP<0.05forhighandlowdoses,respectively.PolysaccharideextractedfrommyceliumofaPhellinussp.wassignificantly(P<0.05)hypoglycemicathigherdoselevelsbutlowerdoseshadnoeffect.
Keywords:
Cordyceps;Phellinus;Ganoderma;polysaccharides;hypoglycemiceffect
MedicinalfungiareaveryimportantpartoftraditionalChineseherbalmedicine.Extensiveresearchonthestructureandbioactivityoffungalcomponentshasindicatedthatfungalpolysaccharidesinparticulararehighlyeffectiveinmaintaininggeneralgoodhealth,andinthetreatmentofcertaindiseasessuchashyperglycemia.Forexample,polysaccharidesextractedfromGanodermalucidum,Cordycepsspp,Coriulusversicolor,Auriculariapolytricha,A.auricula,Tremellafuciformis,T.aurantialba,Hericiumerinaceum,Grifolafrondosa,ArmillariellamelleaandAgrocybepraecoxincreasedtherateofinsulinsecretionandloweredbloodglucoselevels[1,2].WenowreporthypoglycemicfunctionsassociatedwithcrudepolysaccharidepreparationsfromfruitbodiesofG.lucidumandCordycepsmilitaris,andfromvegetativemyceliaofCordycepstaii,CordycepsmemorabilisandPhellinussp.
1MaterialsandMethods
1.1Strainsandtestsample
AllfungalstrainswereprovidedbytheGuangdongInstituteofMicrobiology(GIM).Phellinussp.GIMYY0512wasisolatedfromafruitbodycollectedfromthewildatPreahVihear,Cambodia,andwasidentifiedasanewspeciesofPhellinusthroughITSsequenceanalysis(ITSsequencedepositedinGenbankunderaccessionNo.EU826013);CordycepstaiiGIMYY0508andCordycepsmemorabilisGIMYY0607wereisolatedfromfruitbodiescollectedfromthewildatHenyang,HunanProvinceandDinghu,GuangdongProvince,respectively.FruitbodiesofGanodermalucidumGIMYY0403andCordycepsmilitarisGIMYY0610werecultivatedinourlaboratory.
1.2Testmice
Specificpathogen-free(SPF)NIHmice(maleandfemale,18~22g)weresuppliedbytheGuangdongMedicalandLaboratoryAnimalCenter,LicenceNos.SCXK(Yue)2003-002and2007A004.
1.3Reagentsandmedia
AlloxanwasfromAcrosOrganics,NewJersey,USA,Lot:
A011257301;metforminhydrochloridewasfromShenzhenChinaAssociatedPharmaceutical,Lot:
0610304;Glucosemono-reagentwasfromShanghaiKexinBio-technologyInstitute,Lot:
20070202.
Liquidmedium1(%w/v):
glucose5,peptone1,KNO30.2,MgSO40.2,CaCl20.01,and(NH4)?
?
3PO4?
3H2O0.2;pHadjustedto7.0with1mol/LNaOH.
Liquidmedium2(%w/v):
wheatbran3,glucose3,peptone0.3,yeastextract0.1,K?
?
2HPO40.1,andMgSO40.05;pHadjustedto6.0with1mol/LNaOH.
1.4Cultivationoffungalmycelia
Liquidmedium1(200mL)in500mLflaskswasinoculatedseparatelywith2%~5%(w/v)C.taiiGIMYY0508orC.memorabilisGIMYY0607myceliumandincubatedat26℃for5dwithshaking(120r/min).Myceliawereharvestedbyfiltrationanddriedat50℃.
Liquidmedium2(200mL)in500mLflaskswasinoculatedwith15%~20%(w/v)Phellinussp.GIMYY0512myceliumandincubatedat28℃for7~10dwithshaking(120r/min).Myceliawereharvestedbyfiltrationanddriedat50℃.
1.5Preparationofcrudepolysaccharides
Dryfruitbodiesormyceliaweregroundseparatelyintoapowderandsuspendedin30volwater.Afterextractionfor2hat100℃,thesuspensionwascentrifuged(1400g,10min)andthesolidresiduere-suspendedin20volswaterandextractedagainusingthesameprocedure.Combinedsupernatantswereconcentrated10-foldundervacuum,fourvolumesof90%(v/v)ethanolwereadded,andthemixturewasmaintainedat4℃overnighttoallowcompleteprecipitationofthepolysaccharides.Theprecipitateofcrudepolysaccharidewascollectedbycentrifugation(1400g,15min)anddriedat50~60℃.Thecarbohydratecontentwasdeterminedusingthephenol-sulfuricacidmethod[3],andthepolysaccharideyield(%)=weightofcrudepolysaccharide/dryweightoffruitbodiesormycelia.
1.6Preparationofdiabeticmice
Micewerefedanormaldietfor3daysandthengivenonlywaterfor24hours.Alloxan(0.02g/mL)wasinjectedintotheabdominalcavityatadosageof200mg/kg.Bloodsugarlevelsweredetermined5dayslaterbeforethemicehadbeenfed,andanimalswithavalue>10.0mmol/Lwereselectedasdiabeticmice.
1.7Micetreatmentgroups
Tenuntreatedmiceweredesignatedasthe‘normal’group.Diabeticmiceweredividedrandomlyinto12groups(10miceineachgroup)thatincludedanegativecontrolgroup(administered10mL/kgsaline),apositivecontrolgroup(administered250mg/kgmetforminhydrochloride),andtentestgroupsadministeredeitherhigh(1g/kg)orlow(0.5g/kg)dosesofthedifferentcrudepolysaccharidepreparations.Stocksolutions(preparedinsaline)containingeither1.0g/10mLor0.5g/10mLofcrudepolysaccharidewereusedtoadministerhighandlowdoses,respectively.Treatmentswereadministeredthroughstomachfilling(10mL/kg)every24hfor7daysalongwithnormalfeedandwater.Afterthefinalstomachfilling,micewerefastedfor24handbloodsamplesweretakenfromeyeveins.Serumwasseparatedandbloodsugarvaluesweredeterminedusingtheglucoseoxidasemethodaccordingtothemanufacturer?
?
sinstructions.
1.8Dataanalysis
AllexperimentaldataweresubjectedtostatisticalanalysisbyExcelsoftwareandthet-test.
2Resultsandanalysis
2.1Polysaccharideyields
PolysaccharideyieldsfromdifferentfungiareshowninTable1(seetheChineseversion).Highestpolysaccharideyields(8.1%)wereobtainedfromC.memorabilismyceliumwhereaslowestyields(2.3%)werefromG.lucidumfruitbodies.
2.2Effectofcrudepolysaccharidepreparationsondiabeticmice
TheeffectsofthedifferentpolysaccharidetreatmentsondiabeticmicearesummarizedinTable2(seetheChineseversion).Allofthesamplesexhibitedhypoglycemicfunctionsthatweremorepronouncedinthehigherdosagegroups.CrudepolysaccharideextractedfrommyceliaofC.taiiandC.memorabilisweremosteffectiveinloweringserumglucoselevels.TheimpactsofbothhighandlowdosagesweresignificantatP<0.01,andglucoselevelsinboththehigherdosagegroupsweresimilartothelevelobservedinthepositivecontrolgrouptreatedwithmetformin,acommonmedicinefordiabetics.Incomparison,relativelyweakeffectswereobservedwithcrudepolysaccharideextractedfromfruitbodiesofG.lucidumandC.militaris;theimpactofhighandlowdoseswassignificantatP<0.01andP<0.05,respectively.NosignificanteffectcomparedwithnegativecontrolswasobservedwiththetestgroupgivenlowdosesofcrudepolysaccharideextractedfrommyceliumofPhellinussp.althoughtherewasasignificantimpact(P<0.05)inthegroupadministeredhigherdosesofthismaterial.
3Discussion
Thecytotoxinalloxandamagespancreaticβ-cellsandiswidelyusedtoinduceexperimentaldiabetesinanimalsinordertotestdifferentcompoundsforhypoglycemicfunctions.Inthepresentstudy,lowerdosages(0.5g/kg)ofcrudepolysaccharideextractedfromG.lucidumloweredbloodglucoselevelsby9.3%inalloxan-induceddiabeticmicecomparedwithcontrolsafteroneweekoftreatment.ThesefindingsareinagreementwithpreviousreportsbyCHENetal[4],andbyZHANGandFENG[5].However,ourfindingthatcrudepolysaccharidefromC.militarisatdosesof1.0g/kgloweredglucoselevelsby11.3%didnotcomplywiththeresultsofZHANGetal.[6],althoughthismayhavebeenduetodifferencesinthefungalstrainand/ortheanimalmodeluseintheirinvestigation.
C.taii,whichcontainsvariouschemicalcomponentsincommonwithC.sinensis[7~9],haspreviouslybeenreportedtopossessimmunomodulatoryfunctions[10,11],
whereasC.memorabilishasbeenassignednervecellprotectingproperties[12,13],aswellasantisepticandcytotoxicactivity[14].However,asfarasweareaware,thisisthefirstreportofhypoglycemicfunctionsassociatedwithcrudepolysaccharidematerialextractedfromthesetwofungi.BothMCTandMCMexhibitedgoodhypoglycemiceffectswhenusedathigherdosages(1g/kg).Ourdatashowedthatbloodglucosewasloweredtosimilarlevelsobservedinmicegivenmetforminhydrochloride,andsuggestthatthesematerialsareworthyoffurtherinvestigation.
References
[1]DUM,ZHANGS.Mechanismofediblefungalpolysaccharideonreducingbloodsugar[J].JournalofMicrobiology,2007,27
(2):
83-87.(inChinesewithEnglishabstract)
[2]SHUNYF.Pharmacologicalactionandapplicationofediblefunguspolysacchaides[J].JournalofMedicineCommunity,2006,4
(1):
29-31.(inChinese)
[3]TAOMH,PANQL,ZHANGWM.Testmethodforpolysaccharidesinmedicialfungi[J].EdibleFungiofChina,2005,24(5):
37-38.(inChinese)
[4]CHENWQ,HUANGJW,LUOLQ,etal.StudiesonpreventiveeffectsofGanodermalucidumpolysaccharide(GLP)againsttheformationofhyperlipidaemiainrats[J].ChineseJournalofGerontology,2005,25(8):
957-958.(inChinese)
[5]ZHANGLZ,FENGL.ResearchingthemechanismofGLP'shypoglycemia[J].FujianMedicalJournal,2004,26(3):
137-140.(in
- 配套讲稿:
如PPT文件的首页显示word图标,表示该PPT已包含配套word讲稿。双击word图标可打开word文档。
- 特殊限制:
部分文档作品中含有的国旗、国徽等图片,仅作为作品整体效果示例展示,禁止商用。设计者仅对作品中独创性部分享有著作权。
- 关 键 词:
- Crude Polysaccharides Extracted From Several Medicinal Fungi Exhibit Hypoglycemic Functions in Mice
链接地址:https://www.bdocx.com/doc/7225816.html