Cytotoxic drugs lowered extracellular regulated protein kinase inhibit liver cancer SMF7721 cells pr.docx
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Cytotoxic drugs lowered extracellular regulated protein kinase inhibit liver cancer SMF7721 cells pr.docx
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CytotoxicdrugsloweredextracellularregulatedproteinkinaseinhibitlivercancerSMF7721cellspr
CytotoxicdrugsloweredextracellularregulatedproteinkinaseinhibitlivercancerSMF7721cellsproliferation
[Keywords]livertumorKeywords:
livertumors;proteinkinases;cellcycle;celldivisionAbstract:
ObjectiveTostudythethreecytotoxicdrugs(Harringtoninealkali,vincristine,footleafsaponinsinhibitlivercancerSMF7721cellproliferation,intheprocessofblockingthecellcycle,extracellularregulatedproteinkinase(extracelluarregulatedproteinkinasesofERK1/2phosphorylatedproteinexpressionlevelschangetoexplorecytotoxicdrugsanti-oflivercancerSMF7721themechanismofaction.MethodsMTTassayproliferationinhibitionrateofcellcyclewasdetectedbyflowcytometry,usingWesternblotmethodobservedERK1/2phosphorylationproteinexpressionlevelschangesinresultsofthreecytotoxicdrugs(Harringtoninealkali,vincristine,footleafsaponinscanthedifferentdegreestosuppressSMF7721cellsproliferationinhibitionrateswere(28+-8%,(25+-16%and(24+-11%;HarringtoninealkalitoenabletheSMF7721cellcyclearrestattheG1phase,vincristineitInhibitioninSandG2/Mphase,Vp16sofrustratedatG2/MphasebythreecytotoxicdrugsroleintheactivationstatehepatomaSMF7721intracellularphosphorylationofERK1/2proteinlevelsdecreased.ERK1werethecontrolgroup,43%,32%,72%,ERK2,respectively29%ofthecontrolgroup,20%,61%.conclusionERKpathwaymaybecellthepoisonsthemechanismofactionofanti-hepatomacellsSMF7721one.
Keywords:
liverneoplasms;proteinkinases;cellcycle;celldivision
Abstract:
AIMTostudythechangeofphosphorylated(ac-tivatedERK1andERK2proteinexpressioninthecourseofinhibitinghepatocarcinomatouscellproliferationandarrestingcellcycleprogressionbythreekindsofcytotoxicdrugs,Har-ringtonine(HRT,Vincristine(VCR)andEtoposide(Vp16),andtodiscussthekillingmechanismsofcytotoxicdrugstohepato-carcinomatouscell,SMF7721.METHODSProliferationinhibitionratewasdetectedbyMTTmethod.Thecellcyclewasanalyzedbyflowcytometry.ThechangeofthephosphorylatedERK1/2proteinexpressionwasob-servedbyWesternblotmethod.RESULTSThreekindsofcytotoxicdrugs(HRT,VCRandVp16inhibitedhepatocar-cinomatouscellproliferationtosomeextent.Theinhibitionratewas(28+-8%,(25+-16%and(24+-11%respective-ly.ThecellcyclewasblockedinG1phasewithHRT.ThecellcyclewasblockedinSphaseandG2/MphasewithVCR.AndthecellcyclewasblockedinG2/MphasewithVp16.Inexperimentalgroups,thecontentsofphosphorylatedERK1andERK2proteinwhichwasactiveinhepatocarcinomatouscellSMF7721obviouslydeclined.Withtheeffectsofthreecytotoxicdrugs,thecontentofERK1was43%,32%and72%respectively;thecontentofERK2was29%,20%and61%respectively.CONCLUSIONItmightbethroughERKsignaltransductionpathwayforcytotoxicdrugstoinhibithepatocarcinomatouscellSMF7721proliferationandarrestcellcycleprogression.
0Introduction
Theextracellularregulatedproteinkinase(extracelluarregulatedproteinkinases,ERKpathwayandmalignantphenotypetransitioniscloselyrelatedtotheblockingoftheERKpathwaywillhelptoinhibitthegrowthofcancercells.Selectedseveralcommonlyusedcytotoxicdrugs,toexploretheroleofoneofthemechanismsofregulationoftheERKpathwayiskillinglivercancercellstocytotoxicdrugs.
1Materialsandmethods
Cited1.1MaterialsSMF7721cellsfromWuhanUniversityTypicalCultivationCenter.Containing100mL·L-1FBS,1×10-5U·L-1penicillin,1×10-5g·L-1streptomycintheRRMI1640mediumat37°C,50mL·L-1CO2saturatedhumidityincubatorculturemediumwaschangedevery2~3dpassagedexperimentalselectionofcellsinthelogarithmicgrowthphasethreeharringtonine(harringtonineHRT:
BeijingUnionMedicalCollegepharmaceuticalproduct,lotnumber971191;enoughleafsaponins(etoposide,Vp16:
LianyungangHengRuiPharmaceuticalCo.,Ltd.,batchnumber990504;vincristine(vincristine,VCR:
ShanghaiHualianPharmaceuticalCo.,Ltd.,batchnumber991001;tetramethyltetrazoliumsalt(MTT),dimethylsulfoxide(DM-SO,propidiumiodide(PI,RNaseA.areSigmaproducts.fetalcalfserum(FCSRPMI1640mediumfromGibcoCompany;anti-phosphorylatedERK1/polyclonalantibodywaspurchasedfromSantaCruz.
1.2Methodsweredividedintofourgroups:
plusdruggroup;HRTgroup;VCRgroup;Vp16groupfinalcelldensityof1×109·L-1,thetotalvolumeoftheexperimentalsystem180μL.At37°C,50mL·L-1ofCO2saturatedhumidityincubatorafter24hculture,centrifugationsupernatantwasadded(5g·L-1MTT20μLAfterculturewascontinued4h,coupledDMSO200μLterminatethereaction,usingtheBio-RadM450microplatereader(wavelength540nmmeasuredtheabsorbance(AvalueDrugfinalconcentration:
HRT5×10-4g·L-1,theofVCR2×10-4gL-1,Vp164×10-3gL-1.calculatedasfollowsdrugcellinhibitionrate[1]:
Theinhibitoryrate(%=(1-experimentalwellsAvalue÷controlholeAvalue×100%.respectivelywiththeabove-mentionedconcentrationofHRT,VCRs,,andVp16processingSMF7721cellline24htake1×10
6cells,PBSwashed3timeswith700mL·L-1ethanol,RNaseA(5×10-5g·L-1digest30min,PI(0.5g·L-130μL,darkfor30minFlowcytometricanalysisofcellularDNAcontentdistributioninformationbyModFitLTRsoftwarecollection,storageandanalysis[2]totakethedrugaction1×107cells,addingthepre-coolingofthecelllysate200μLoficecracking20mincentrifugal,thesupernatant,751spectrophotometersmeasuringproteincontent,eachsampletaken30μgprotein100mL·L-1SDS-polyacrylamidegelelectrophoresisandtransferredtonitrocellulosemembrane.thefilmby50mL·L-1nonfatdrymilkclosed,andtheantiofERK1/2phosphorylationantibodieswereincubatedovernightwithalkalinephosphataselabeledsecondaryantibodyandBCIP/NBTsubstratecolorimageacquisitionUvpgrab-itImagelsoftware,theGelworks1DAd--vancedv4.of01softwareprocessing[3].
2Results
2.1SMF7721cellproliferationHRT(5×10-3g·L-1,VCR(1×10-3g·L-1andVp16(4×10-3g·L-1role24habledegreeofinhibitionSMF7721cellproliferationinhibitionrateswereasfollows:
(28+-8%(25+-16%(24+-0.11%.
Cytotoxicdrugsfor24h2.2cellcyclearrest,thecellcyclebyblock,which,HRTrepressorcellsinG1phase,VCRInhibitorycellsinSphaseandG2/Mphase,Vp16sofrustratedattheG2/Mphase(Tab1.
Table1cytotoxicdrugsslightlyimpactofthehepatomacellSMF7721cellcycle
2.3cytotoxicdrugsinhibitionofERK1/2phosphorylationHRTVCRandVp16roleof24h,SMF7721intracellularERK1/2phosphorylationproteincontentdecreased;usingGelworks1DAdvancedv4.01softwareimagegrayvalueofeachtestgroupanalysis,ERK1,respectively,forthecontrolgroup,43%,32%,72%,ERK2,respectively,for29%ofthecontrolgroup,20%,61%,suggestingthatthereducedphosphorylatedERK1/2proteinexpression(Fig1.stateofphosphorylationofERK1/2proteinexpressionlevelsmayindirectlyreflectofERK1/2functionalactivityofhighandlow.
LinkstofreepapersDownloadCenter
Figure1slightly
3Discussion
ERKismostclosetothemembersoftheRas/Raf/MEK/ERK1/2signalpathwayandthenucleus,belongtothemitogenactivatedproteinkinase(mitogen-activatedproteinkinase,MAPKfamily,bothnormalandmalignantcellsbycertainexternalstimuli,canbeactivatedbyphosphorylationofERK1/2,translocationfromthecytoplasmtothenucleus,therebymediatedElk-1,theATF,NF-κBandAp-1,c-fosandc-juntranscriptionalactivationisinvolvedincellmultiplebiologicalresponses,suchasdifferentiationandapoptosis[4,5].acuteleukemiaandrenalcellcarcinomaandmalignantdiseasewerefoundERKbuildactivation,suggestingthatactivationoftheERKpathwaymaybeinvolvedintheprocessofmalignanttransformationofnormalcells[6].Boucheretal[7]studyconfirmedtheactivationoftheERKpathwaymaypreventapoptosisofpancreaticcancercellsandpromotecellcycleprogression.Kangetal.[8]studyconfirmedtheanti-apoptoticpropertiesoftheleukemiacelllineK562andERKbasalactivityinhibitionofERKactivityallowsK562apoptosis.visibleERKpathwayinthedevelopmentofmalignantcellsplaysanimportantroleinERKactivitycutwillhelpinhibitcancercellgrowthanddevelopment.,cytotoxicdrugskillingThetumorcellsmaybeonemechanismisthatinhibitionoftheERKpathway.
Toconfirmthisinference,weselectedexperimentshepatomacelllineSMF7721astargetcells,threekindsofinteractioncharacteristicsofdifferentcytotoxicdrugsofHRT,VCRandVp16asaninducerofERK1/2inthedestructionoflivercancercytotoxicdrugscells.,HRTcyclenon-specificdrugs;VCRMphasecyclespecificdrugs;Vp16SphaseandG2phasecyclespecificdrugsTheresultsshowthatthephosphorylationofofcytotoxicdrugsHRT,VCRandVp16abletocutSMF7721cellsstatusofERK1/2proteinexpressionlevels;alsoallowscellproliferationwasinhibitedbyblockingcellcycleprogression.Basedonthis,webelievethatthemolecularmechanismsoftheanti-hepatomacellsSMF7721ofthesethreekindsofcytotoxicdrugsmaybebyinhibitionoftheRas/Raf/MEK/ERK1/2signalpathway,reducingERKactivity,reducethetranscriptionalactivationofERK1/2targetgenesinhibitcellproliferation,arrestcellcycleprogressioninshort,inthetreatmentoflivercancerprocess,ERK/MAPKpathwaymaybecytotoxicTargetsofDrugsimportantrole.
References:
[1]ZhaoJB,CuiQ,ZhangX,WangWL,JiangYP.Experimentstudyonanticanceractiono
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