所有质粒载体汇总.docx
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所有质粒载体汇总.docx
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所有质粒载体汇总
酿酒酵母表达载体
pYES2,pYES2/NT,pYES2/CT,pYES3,pYES6,pYCplac22-GFP,
酵母载体pAUR123,pRS303TEF,pRS304,pRS305,pRS306,pY13TEF,pY14TEF,pY15TEF,pY16TEF,
酵母基因重组表达载体pUG6,pSH47,
酵母单杂载体pHISi,pLacZi,pHIS2,pGAD424, 酵母双杂交系统:
酿酒酵母Y187,
酿酒酵母AH109;质粒pGADT7,pGBKT7;对照质粒pGBKT7-53,pGBKT7-lam,pGADT7-T,PCL1,
酿酒酵母菌株INVSc1,YM4271,AH109,Y187,Y190,
毕赤酵母表达载体pPIC9K,pPIC9K-His,pPIC3.5K,pPICZalphaA,B,C,pPICZA,B,C,pGAPZαA,pAO815,pPIC9k-His,pHIL-S1,pPinkhc,
配套毕赤酵母Pichiapink,
毕赤酵母宿主X33,KM71,KM71H,GS115,
原核表达载体pQE30,31,32,40,60,61,62,等原核表达载体,包括pET系列,pET-GST,pGEX系列(含GST标签),pMAL系列pMAL-c2x,-c4x,-c4e,-c5x,-p5x,pBAD,pBADHis,pBADmycHis系列,pQE系列,pTrc99a,pTrcHis系列,pBV220,221,222,pTXB系列,pLLP-ompA,pIN-III-ompA(分泌型表达系列),pQBI63(原核表达带荧光)pET3a,pET3d,pET11a,pET12a,pET14b,pET15b,pET16b,pET17b,pET19b,pET20b,pET21a,b,d,pET22b,pET23a,pET23b,pET24a,b,pET25b,pET26b,pET27b,pET28a,b,pET29a,pET30a,pET31b,pET32a,pET35b,pET38b,pET39b,pET40b,pET41a,bpET42a,pET43.1a,bpET44a,pET49bpET302,303pETHis,pETDsb,pETGST,pETTrxpQE2,pQE9pQE30,31,32,pQE40pQE70pQE80LpQETirssystempRSET-ApRSET-BpRSET-CpGEX4T-1,-2,-3,5x-1,6p-1,6p-2,2tk,3cpBV220,221,222pTrcHisA,B,CpBAD24,34,43pBADHisA,B,CpPinPoint-Xa1,Xa2,Xa3pMALc2x,p2xpBV220pGEMEx1,pGEM7ZF(+),pTrc99A,pTwin1,pEZZ18pkk232-8,pkk233-3,pACYC184,pBR322,pUC119pTYB1,pTYB2,pTYB4,pTYB11pBlueScriptSK(+),pBlueScriptSK(-)pLLPompA,pINIIIompA,pMBP-P,pMBP-C,
大肠杆菌冷激质粒:
pColdIpColdIIpColdIIIpColdTF 原核共表达质粒:
pACYCduet-1,pETduet-1,pCDFduet-1,pRSFduet-1Takara公司大肠杆菌分子伴侣:
pG-KJE8pGro7pKJE7pGTf2pTf16 大肠杆菌宿主细胞:
DH5aJM101JM103JM105JM107JM109JM110Top10Top10FBL21(DE3)HB101ER2529E2566C2566MG1655XL-10goldXLblueM15JF1125K802SG1117BL21(AI)BL21(DE3)plysSTG1TB1DH5a(pir)Tuner(DE3)Bl21codonplusRIPLNovablue(DE3)RosettaRosetta(DE3)Rosetta(DE3)plysRosetta-gami(DE3)Rosetta-gamiB(DE3),Rosetta-gamiB(DE3)plysSOrgami(DE3)OrgamiB(DE3)HMS174(DE3)
植物表达/RNAi载体农杆菌pBI121,pBI121-GFP,pBI101,pBI221,pSN1301,pUN1301,pRTL2,pRTL2-GFP,pRTL2-CFP,pRTL2-RFP,pRTL2-YFP,pCAMBIA1300,1301,1302,1303,1304,1305,1381Z,1391Z,2300,2301,3300,3301,pCAMBIAsuper1300,pCAMBIAsuper1300-GFP,pPZP212,pPZP2121,pPZP212-GFP,pGDG,RNAi
载体pART27,pHANNIBAL,pKANNIBAL,pFGC5941,pTCK303,pTRV1,pTRV2,T-DNA插入载体(随机突变体库)pSKI015,pSKI074,真菌ATMT载体pBIG2RHPH2-GUS-GFP,pBHt1
枯草芽孢杆菌表达载体pWB980,pHT43,pHP13,pHP43,pBE2,pMUTIN4,pUB110,pE194,pMA5,pMK3,pMK4,pHT304,pHY300PLK,pBest502,pDG1363,pSG1154,pAX01,pSAS144,pDL,pDG148-stu,pDG641,pAL12,pUCX05-bgaB,pHT01, 配套菌株BS168,WB600,WB800,WB700,WB800N,1012,FZB42,1A747,广宿主质粒pVLT33
RNAi基因沉默干扰敲除载体pSilencer1.0,pSilencer2.1-U6hygro, pSilencer3.1-H1hygro,pSilencer3.1-H1neo, pSilencer4.1-CMVneo, pSilencer4.1-CMVpuropMIR-REPORTLuciferaseRNAi载体(oligoengine)pSuper-puroRNAi逆转录病毒载体(clontech):
RNAi-ReadypSIREN-RetroQ, RNAi-ReadypSIREN-RetroQ-ZsGreen(LuciferaseshRNAAnnealedOligonucleotide)RNAi慢病毒载体(addgene):
pLKO.1
哺乳动物表达载体pcDNA3.1+/-,pcDNA4/HisMaxB,pSecTag2A,pVAX1,pBudCE4.1,pTracerCMV2,pcDNA3.1(-)/myc-HisA ,pcDNA6-Myc/HisB,pCEP4, pIRES,pIRESneo,pIREShyg3,pCMV-myc,pCMV-HA,pIRES-puro3,pIRES-neo3,pCAGGS哺乳动物双杂交系统pACT,pBIND,pACT-MyoD,pBIND-Id,pG5luc,pCMV-BD,pCMV-AD,pBD-p53,pFR-luc,CytotrapTwo-HybridSystem:
pSos,pSosMAFB,pMyr
蜕皮激素诱导系统pIND,pVgRxR,LacSwithII哺乳动物诱导表达系统:
pOPRSVI ,pOPI3CAT,pCMVLacI,GeneSwitchSystem:
pSwitch哺乳动物表面展示系统:
pDisplay, 四环素调控系统(Invitrogen):
pcDNA4/TO/Myc-HisA,pcDNA4/TO/Myc-HisB,pcDNA4/TO/Myc-HisC,pcDNA4/TO/Myc-His/LacZ,pcDNA6/TR四环素调控系统(Clontech):
pTet-On,pTet-Off,pTRE2,pRevTRE,pRevTet-On,pRevTet-off信号通路报告载体:
pGAS-TA-Luc,pSTAT3-TA-Luc,pISRE-TA-Luc,pTA-Luc,pIκB-EGFP,pNFAT-TA-Luc,pCaspase3-sensor,pAP1(PMA)-Luc;pGL4.26[luc2P/minP/Hygro],pGL4.29[luc2P/CRE/Hygro],pGL4.30[luc2P/NFAT-RE/Hygro],pGL4.75;p53-Luc,pAP-1-Luc, pNF-κB-Luc,pSRE-Luc,pFA2-Elk1,pFC-MEKK,pFR-luc,Gateway系统(invitrogen)pcDNA6.2-GWEmGFP-miRnegative,pLenti6/TR,pcDNA6.2-GWEmGFP-miR,
乳酸菌表达载体及各种乳酸菌乳酸杆菌菌株,pNZ8148,pLEISS,pMG36e,pBBR1MCS-5,pBBR1MCS-6,pRV610,pLEM415,pHY300PLK,分泌型乳酸菌表达载体pVE5523,pPG611.1,pPG612.1等和乳酸杆菌菌株宿主菌NZ9000,MG1363,Lactobacilluscasei1.539,Lactobacilluscasei,acidophilusNCFM,1.2,Lactobacillussakei23K,L.plantarum,L.rhamnosusGG,B.coagulans,Bifidobacteriumbifidum,Bifidobacteriuminfantis,LactococcuslactisM17,1663,Lactobacillusreuterii
广宿主表达载体链球菌表达敲除载体假单胞菌表达载体pVLT33,pBBR1MCS-2,3,4,5,6,pJRD215,pJN105,pME6032,Cos载体pLAFR3,pMP2444(GFP),pHY300PLK,pRT102,pRL1063a, 转座子载体pUT-miniTn5,pMGS100,pWHM10,pKC1139,pSET152,pOJ260,pPG611.1,pPG612.1,
腺病毒载体/慢病毒,逆转录病毒表达载体及包装包膜质粒,腺病毒系统(Stratagene):
pAdEasy-1,pShuttle-CMV,pShuttle,pAdTrack,pAdTrack-CMV,pShuttle-IRES-hrGFP-1、pShuttle-IRES-hrGFP-2、pShuttle-CMV-lacZ,pShuttle-CMV-EGFP-C,pXC1,pBHGE3, 配套大肠杆菌BJ5183,293,293Tcellline 腺相关病毒系统(Stratagene):
pAAV-MCS,pAAV-RC,pHelper,pAAV-LacZ,pAAV-IRES-hrGFP,pCMV-MCS, 慢病毒载体:
pLVX-DsRed-Monomer-N1,pLVX-IRES-ZsGreen1,pLVX-AcGFP1-N1,Lenti6/v5-EDST-EGFP,pWPXL,FUGW,pLentilox3.7,RNAi-ReadypSIREN-RetroQ,RNAi-ReadypSIREN-RetroQ-ZsGreen,pSUPER.Retro-GFP/Neo,pSUPER-Retro-Neo,pSUPER.Retro-puro,PLNCXPLNCX2pMSCV-HYGpMSCV-neopMSCV-puropLEGFP-C1pLOX-CW-CREpLOX-GFP-IRES-TKpRetroX-IRES-DsRedExpress,pLVX-IRES-mCherry质粒载体。
病毒包装:
二质粒包装系统一:
psPAX2pMD2.G 二质粒系统二:
pCMV-dR8.2dvprpCMV-VSVG 三质粒包装系统具有更高的包装效率:
pMDLg/pRREpRSV-RevpMD2.GInvitrogen包装系统:
PLP1PLP2PLP/VSVG
基因重组载体敲除载体自杀质粒RED重组系统载体pKD3,pKD13,pKD4,pCP20,pKD46,pCVD442,pk18mobsacB,p2NIL,pMAD(革兰氏阳性菌基因敲除
),pBT2,RN4220,pKC1139,pOJ260,pJQ200SK,pSET152,pWHM10,pk18mobsacB,pGOAL17,pGOAL19,基因打靶载体PL253,PL451,PL452,PL453,配套菌株EL250,EL350,pMLM290,292,KJBAC1,pST1374
荧光蛋白表达载体荧光定位质粒报告基因载体pEGFP,pEYFP,pECFP,pDsRED,pmCherry,pAcGFP,hrGFP,ZsGreen,pDsRed1-C1,pDsRed1-N1,pDsRED-Monomer-N1,pEYFP-C1,pEYFP-N1,pEGFP-1,pEGFP-C1,pEGFP-N1,pEGFP-N3,pcDNA-EGFP1,pECFP-N1,pECFP-C1,pAcGFP1-1pDsRED2-ER,pDsRED2-Mito,pDsRED2-Nuc,pDsRED2-Peroxi,pEYFP-Golgi,pEYFP-Mem,pEYFP-ER,pEGFP-Actin,pECFP-ER,pIRES2-EGFP,pLEGFP-C1,pLEGFP-N1,pDsRED-Express-N1,pIRES2-DsRed,pIRES-AcGFP1,pmCherry-C1,N1 启动子增强子报告载体(Promega,带有Luc荧光报告基因):
pGL3-basic,pGL3-control,pGL3-enhancer,pGL3-promoter,pRL-TK,pRL-CMV,pRL-SV40, 信号通路报告载体(Clontech):
pGAS-TA-Luc,pSTAT3-TA-Luc,pISRE-TA-Luc,pTA-Luc,pIκB-EGFP,pNFAT-TA-Luc,pCaspase3-sensor,pAP1(PMA)-Luc 信号通路报告载体(Promega):
pGL4.26[luc2P/minP/Hygro],pGL4.29[luc2P/CRE/Hygro],pGL4.30[luc2P/NFAT-RE/Hygro],pGL4.75 信号通路报告载体(Stratagene):
p53-Luc,pAP-1-Luc,pNF-κB-Luc,pSRE-Luc, pFA2-Elk1,pFC-MEKK,pFR-luc
昆虫表达载体杆状病毒表达载体系统pFastBac1,pFastBac1-Gus,pFastBacHTApFastBacHT-CAT,pFastBacDual,pBlueBacHis2ADH10Bac配套大肠杆菌,pMIBv5-HisB,pIZT/V5-His,
Taq,Pfu高表达载体菌株KOD,T4连接酶高表达质粒载体,高产基因工程菌株、酶基因KOD,T4kinase激酶,Tth反转录酶MMLV,KlentaqPCR酶,Cre酶,RNAinhibitor抑制酶,DL2000质粒
BiFC双分子荧光表达载体FRET系统载体pBiFC-VC155,pBiFC-VN173,pBiFC-bFosVC155,pBiFC-bFos(deltaZIP)VC155,pBiFC-bjun-VN173,pCE-BiFC-VN173,pCE-BiFC-VC155,
真菌表达载体黑曲霉米曲霉黄曲霉表达质粒pBC-hygro,pAN7-1,pBARGPE1, 真菌ATMT农杆菌介导转化载体 pBHt1,pBIG2RHPH2-GFP-GUS等,
酵母单杂交三杂交载体,酵母双杂交系统pGBKT7,pGADT7,pCL1,pGBKT7-53,pGADT7-T,pGBKT7-Lam,配套酿酒酵母AH109 酵母单杂交系统(Clontech):
pHISi,pLacZi,pHis2,pHIS2.1 酵母三杂交系统:
pBridge,YBZ1菌株 CytotrapTwo-HybridSystem(Stratagene):
pSos,pSosMAFB,pMyr
噬菌体展示系统载体1.噬菌体展示抗体scFv 质粒:
pCANTAB5E 宿主菌:
TG1 辅助噬菌体:
M13K07 可溶表达菌:
HB21512.噬菌体展示抗体Fab 质粒:
pcomb3 宿主菌:
XL1-Blue 辅助噬菌体:
VCSM13
pEVOL,pBudCE4.1,pBudCE4.1-EGFP,Zeocin,pEGFP-C3,2,1,pmCherry-C1,-N1,pLOVE-miR302/367,pDsRed2-N1,pAcGFP1.1,TALEtoolboxkit,pIRES-hyg3,pIRES-Neo3,pIRES-puro3,pSIREN-RetroQ-ZsGreen,GST14-3-3,pDsRed-Monomer-N1,M50super8xTOPFlash,pRK793,pCMV-tag5B,pCMV-tag2B,pCMV-N-HA,pUAST,pw-25,PAC-5.1-HISA,pSLIK-Neo,pCMV-myc-cyto,pCMV-myc-nuc,疫苗表达载体pVAX1,pAdEasy-1,pShuttle,pAdTrack,pAdTrack-CMV,-polyA,pShuttle-EGFP,BJ5183,pXC1,pBHGE3,pSUPER-retro-GFP-Neo,pORF-lacZ,pORF-TK,pCOX2-Luc,pcDNA3.1(-)-myc-HisA,pcDNA3.1-p300,pINP,pOprE,pCR2.1TOPO,pLP1,pLP2,pLP/VSVG,Stbl3,plenti6/V5-DEST,pLVX-shRNA1,2,pLVX-IRES,tdTomato,FUW-teto-hOCT4,hSOX2,hMYC,hKLF4,FUW-M2rtTA,pSecTag2A,pEYFP-C1,pEGFP-N1,-C1,pcDNA3.1-V5-HisA,B,C,pcDNA3.1-EGFP,pLEGFP-C1,α-MHCPromoter,Marc-145猴肾细胞,Sf9,BmN昆虫家蚕细胞,酵母载体pYES2,pYES2.0,INVSc1,pYES3,pYES6,pRS41H,pRS303,304,305,306,pRS424,425,426,414,415,416,pY13,14,15,16,YBZ1,L40酵母菌,pESC-His,pYIP5,pHIS2,pHIS2.1,pHISi,pLacZi,酵母重组载体pUG6,pSH47,植物载体pCAMBIA1300-EGFP,pTCK303,pTRV1,pTRV2,pFGC5941,pUN1301,pSN1301,C58C1,R1601,AGL1农杆菌感受态,原核载体pQE3,pQE30,31,32,pQE60,61,62,M15,DH10Bac,Origami(B)DE3,XL1-Blue,RosettagamiB(DE3)pLysS,TKB1,ER2566
,DB3.1,JM105,TOP10,DH5a,DH5alphalambdapir,C43(DE3)菌株感受态,pTrcHisA,B,C,pTWIN1,pTYB1,pTYB11,pACYC184,pMAL-c5x,c2x,p5x,c4x,pBV220,221,222,pColdI,II,III,TF,pET22a,pET29a,pET28a,pET39b,pET41a,pET42a,p15A,pET-sumo,pBADHisA,B,C,pBAD24,34,pETDuet-1,pRSFDuet-1,pCDFDuet-1,pACYCDuet-1,原核荧光pQBI63,pMP2444,链球菌表达、敲除载体pKC1139、pOJ260、pWHM3,pSET152,pVA981,多宿主载体pGOAL19,pBBR1MCS-2,-5,-6,pDSK519,pLA2917,pRK2013,pVLT33,pMAD,RN4220金葡菌株,真菌载体pBARGPE1,pAN7-1,pBHt1,pBIG2RHPH2-GFP-GUS,乳酸菌载体MG1363,pNZ8148,NZ9000,pNZ9530,pMG36e,枯草芽孢杆菌载体pHT43,pWB980,pHT300PLK,pMA5,pUTminiTn5,巨大芽孢杆菌表达载体pHIS1525,WH320菌,谷氨酸棒状杆菌表达载体pEC-XK99E,敲除载体pk18mobsacB,
pET载体
3a,3d
3c-sumo
5b
11aA+
12aA+
15bA+
16bA+
17bA+
19bA+
20bA+
21a,21b,21dA+
22bA+
23a,23bA+
23a-tev
24aK+
25bK+
26bK+
27bK+
28a,28b,28cK+
28a-sumo
28a-p53
29aK+
30a,30cK+
31bA+
32aA+
32apelBA+
35bK+
39bK+
40bK+
41a,41bk+
42a,42bK+
43bA+
43.1aA+
44aA+
48bK+
49bK+
50bK+
pETduet-1
pACYCduet-1
pCDFduet-1
pRSFduet-1
pColAduet-1
pETHis
pETDsbA
pETGST
pETTrx
pQE载体
PQE2
pQE9
pQE30,31,32,
pQE30UA
pQE30LacIq
pQE40L
pQE60
pQE70
pQE80L
其他原核表达载体
PBBR1MCSCm+
PBBR1MCS-2K+
PBBR1MCS-3Tet+
PBBR1MCS-4A+
PBBR1MCS-5 庆大霉素
pMalc2x,p2x,5x,5E
pBV220,221,222
pTrc99a,pTrcCKS,pTrcHisA,B,C
pBAD24,33,43,pBADHisA,B,C,pBADmycHisA
pEZZ18
pThioHisAA+
pGEX4T-1,4T-2,4T-3,5X-1,6p-1,2t,3x
pColdI
pColdII
pColdIII
pColdTF
pTwin1
pRsetA,B,C
PLLP-STQ
pINⅢ-ompA
pkk223-3
pSE380
pJLA502
pMBP-C,
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