生物化学期末考试题目.docx
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生物化学期末考试题目.docx
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生物化学期末考试题目
中南大学2003-2004学年第二学期考试试卷
Exampaperforthe2ndtermof2003-2004atCentralSouthUniversity
Subject:
Biochemistry
Specialty:
Bioengineering
Coursecharacter:
Required
Exammode:
Withoutreferencedocumentsbutpermissionofanykindofdictionary
Examtimelength:
120minutes
StudentName_____________,RegisterNo.__________Score____________
PartITrueorFalse(1pointeach,20pts)
PleasewriteT(true)orF(false)intheblanket
1.Passivediffusionneedsenergy()
2.Glucosetransportinerythrocytesoccursbyfacilitateddiffusion()
3.Allactivetransportsystemsareenergy-couplingdevices()
4.Glycolysisisacatabolismtodegradeglucose()
5.Glycolysisprovidetheorganismnotonlyenergybutalsosomeimportantintermediates()
6.TCAcycleisthemajorwaytoproduceenergybyaerobicorganisms()
7.BothphotophosphorylationandoxdativephosphorylationproduceATP()
8.ATPisnotthemajorenergyforminthelivingorganism()
9.ATP,acetyl-CoAandglucose-6-phosphatehavehigh-energybond.()
10.NADHnormallyoccursinbiosynthesispathway,whileNADPHoccursprimarilyinbiodegradationpathway()
11.TCAcycleproducesATPdirectly()
12.Carbohydrateproducedbyphotosynthesisoccursinthedarkperiod()
13.Lipidbilayertakespartinmasstransfer,energyproductionandsignalcommunication()
14.Biochemicalreactionhasnotspacebutsubstratespecifications()
15.Degradationoflipidcanprovidemuchmoreenergyandwaterthancarbohydrates()
16.NADPHisnecessaryformaintainingthereductiveenvironmentsforbloodcells()
17.Prolineoftenoccursinaterminalandturnofα-helixofprotein()
18.Alphahelixandbeta-sheetofproteinisduetohydrogenbond,butsubunitsofhemoglobulinarearrangedduetohydrophobicforce.()
19.Changeinconformationisnormallyaccompaniedbybreakageofchemicalbond(s)()
20.Pre-mRNAtranscriptedfromDNAforprokaryotesnormallydoesnotneedanyprocessingtoformthefunctionalmRNA.()
PartIIMultipleChoice(1pointeach,30pts)
Choosetheonebestanswertoeachofthefollowingquestions.
1.____Biochemicalsynthesisofnewcellmaterialiscalled
A.Metabolism
B.Anabolism
C.Catabolism
D.Synthatabolism
2.____TCAcycleisapathwayof
A.anabolism
B.catabolism
C.photosynthesis
D.reductivepathway
3.____Chemoorganotrophsusewhichofthefollowingasanenergysource?
A.Organiccompounds
B.Inorganiccompounds
C.Bothorganicandinorganiccompounds
D.Light
4.____Afastidiousorganismis
A.oxygensensitive
B.temperaturesensitive
C.nutritionallydemanding
D.atmosphericpressuresensitive
5.____IfG0ispositive
A.thereactionisexergonicandrequirestheinputofenergy.
B.thereactioninendergonicandrequirestheinputofenergy.
C.thereactionisexergonicandenergywillbereleased.
D.thereactionisendergonicandenergywillbereleased.
6.____Whichstatementistrue?
A.Coenzymesaregenerallyboundtightlytotheirrespectiveenzymes.
B.Prostheticgroupsaregenerallyboundtightlytotheirrespectiveenzymes.
C.Coenzymesandprostheticgroupsarebothboundtightlytotheirrespectiveenzymes.
D.Coenzymesandprostheticgroupsaretechnicallynotboundtoanyenzymes.
7.____Inanelectroncarriersystem,thenetenergychangeisdeterminedbythedifferenceinreductionpotentialsbetweenthe
A.primaryelectrondonorandtheterminalelectrondonor.
B.primaryelectronacceptorandtheterminalelectronacceptor.
C.primaryelectronacceptorandtheterminalelectrondonor.
D.primaryelectrondonorandtheterminalelectronacceptor.
8.____TheEmbden-MeyerhofPathwayisanothernamefor
A.thecitricacidcycle
B.glycolysis
C.electrontransport
D.NADHproduction
9.____Inglycolysis
A.thecrucialproductisATP;thefermentationproductsarewasteproducts.
B.thecrucialproductisethanolorlactate;ATPisawasteproduct.
C.thecrucialproductisCO2;ATPisawasteproduct.
D.thecrucialproductisnotrelevantbecauseglycolysisisnotamajorpathway.
10.____Inchemiosmosis
A.OH-accumulatesontheoutsideofthemembranewhileH+accumulatesontheinside.
B.OH-accumulatesontheinsideofthemembranewhileH+accumulatesontheoutside.
C.bothOH-andH+accumulateontheinsideofthemembrane.
D.bothOH-andH+accumulateontheoutsideofthemembrane.
11.____Whichstatementisnottrue?
UncouplerspreventATPsynthesis
A.byinterferingwithelectronflow.
B.bycreatingleakymembranes.
C.withoutaffectingelectrontransport.
D.bydestroyingtheprotonmotiveforce.
12.____Thechemicalsubstancethatentersthecitricacidcycleforfurthermetabolismis
A.ethylalcohol
B.acetyl-CoA
C.adenosinetriphosphate
D.pyruvicacid
13.____Whichofthefollowingoccursintheabsenceofoxygen?
A.Fermentation
B.Anaerobicrespiration
C.Anoxygenicphotosynthesis
D.Alloftheabove.
14.____Mostchemolithotrophsare
A.phototrophs
B.autotrophs
C.heterotrophs
D.organotrophs
15.____Foracarbonsource,chemoorganotrophsgenerallyusesuchcompoundsas
A.glucose
B.carbondioxide
C.triglycerides
D.sunlight
16.____Foreachmoleculeofpyruvatethatentersthecitricacidcycle,howmanymoleculesofCO2aregenerated?
A.1
B.2
C.3
D.6
17.____Whetheranorganismisclassifiedasaphotoheterotrophoraphotoautorophdependsonits
A.energysource.
B.carbonsource.
C.oxygenrequirements.
D.Alloftheabove.
18.____HowmanyATPresultfromthetransferofelectronsfromNADHtoO2throughtheelectrontransportchain?
A.1
B.2
C.3
D.6
19.____Todescribeatypeofphotosynthesisas"oxygenic"impliesthat
A.oxygenisproduced
B.oxygenisconsumed
C.oxygenfunctionsasacatalyst
20.____TheCalvincycle
A.isresponsibleforthefixationofCO2.
B.utilizesonlyATP.
C.requiresphosphofructokinaseandpyruvatedecarboxylase
D.alloftheabove
21.____DNAisalwayssynthesizedinthe____direction.
A.aminoterminaltocarboxyterminal
B.carboxyterminaltoaminoterminal
C.3'to5'
D.5'-5'
E.5'-3'
22.____TheshortpiecesofDNAmadeinthediscontinuousstrandarecalled______fragments?
A.Intron
B.Cistron
C.Exon
D.Polycistronic
E.noneofthese
23.____DNAreplicationbeginsatlocationscalled___sites.
A.Promoter
B.Ori
C.Shine-Delgaro
D.Okazaki
E.Intron
24.____AllDNApolymerasesneeda______toattachanucleotideto.
A.RNAprimer
B.Ligase
C.3'-OH
D.deoxynucleotide
E.5'-triphosphate
25.____WhichofthefollowingisnotasubstrateforDNApolymerases?
A.deoxyadenosinetriphosphate
B.DNAtemplate
C.anucleotideprimerwithafree3'-OH
D.deoxyuraciltriphosphate
E.deoxyguanosinetriphosphate
26.____WhichofthefollowingreplacestheRNAprimerwithdeoxynucleotides?
A.DNApolymeraseI
B.DNApolymeraseII
C.DNApolymeraseIII
D.DNAligase
E.primase
27.____IfVmax=100μmol/mLsecandKm=2mM,thevelocityofthereactionis______,when[S]=20mM
A.108μmol/mLsec
B.91μmol/mLsec
C.67μmol/mLsec
D.145μmol/mLsec
28.____Thecarboxylationcatalyzedbyacarboxylaseusuallyneedsaprotheticgroup
A.Fe2+
B.biotin
C.ferrocyanin
D.Thiamine
29.____Inatypicalcycleofbeta-degradationofC16saturatedfattyacids,itincludestwostepsofoxidation,inwhichtheelectroncarriersarerespectively:
A.FADandNAD+
B.NAD+andFAD
C.FADandFAD
D.NAD+andNAD+
30.____Inanerythrocyteundergoingglycolysis,whatwouldbetheeffectofasuddenincreaseintheconcentrationofATP
A.Glycolysisinhibited
B.glycolysisstimulated
C.fructose-1,6-bisphosphateaccumulated
D.acetyl-CoAaccumulated
PartIIIFillingBlankets(1pointeach,20pts)
Pleasefilleachblanketwithsuitableword(s)eitherinEnglishorChinese
1.Whenexternalelectronacceptorsarenotavailableinanoxicenvironments,carboniscatabolizedby(_____________________).
2.Thetwomajorproductsofpentosephosphatepathwayare(_______________________)and
(___________________________)
3.Themajorchemicalenergyformsfromcatabolismthatareusedforbiosynthesisare(_________________________)and(___________________________).
4.TherearesixsubclassesofenzymesaccordingtotheEnzymeCommission,pleasegivetheirsystematicnames(_______________________________;___________________________,
_________________________________,______________________________;
_________________________________;_______________________________)
5.Thefatesofpyruvateproducedbyglycolysisare:
(1)_______________________________;
(2)_________________________
(3)_______________________________;(4)_________________________
6.Theenergysourcefortheheterotrophs,phototrophsandlithotrophscorrespondinglyare
(_________________________________________;______________________________;
__________________________________________).
7.Besidesglycolysis,pleaselisttheothertwomostimportantpathways:
(___________________________________;____________________________________)
PartIVShortessayquestions(30pts)
PleaseanswerthefollowingshortessayquestionseitherinEnglishorChinese
1.Pleasedescribethestructuralcharactersofthefourdifferentlevelsofprotein.(4pts)
2.Pleasecompareandcontrastfermentationandrespiration.(4pts)
3.Chemiosmoticisusedtoexplainthemechanismofphosphorylation(seethefollowingfigure).Pleaseexplain.(4pts)
ATP,32P
labeled
4.PleasedescribetheregulationoftheTCAcycleaccordingtothefollowingfigure(4pts)
5.Theglyoxylatecycleofplantsandbacteria(seefigurebelow)areveryimportantfortheirseedling,Pleaseexplain(4pts).
6.Describethecomplexesa
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