AATCC-100-2004.docx
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AATCC-100-2004.docx
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AntibacterialFinishesonTextileMaterials:
Assessmentof
Developedin1961byAATCCCommitteeRA31;revised1965,1981,1988(withtitlechange),1993,1999;editoriallyrevised1969,1971,1974,1985;reaffirmed1977,1981,1989,1998;editoriallyrevisedandreaffirmed1986,2004.
1.PurposeandScope
1.1Thistestmethodprovidesaquantitativeprocedurefortheevaluationofthedegreeofantibacterialactivity.Assessmentofantibacterialfinishesontextilematerialsisdeterminedbythedegreeofantibacterialactivityintendedintheuseofsuchmaterials.Ifonlybacteriostaticactivity(inhibitionofmultiplication)isintended,aqualitativeprocedurewhichclearlydemonstratesantibacterialactivityascontrastedwithlackofsuchactivitybyanuntreatedspecimenmaybeacceptable.However,ifbactericidalactivityisintendedorimplied,quantitativeevaluationisnecessary.Quantitativeevaluationalsoprovidesaclearerpictureforpossibleusesofsuchtreatedtextilematerials.
2.Principle
2.1SwatchesoftestandcontroltextilematerialsaretestedqualitativelyforantibacterialactivitybyAATCCMethod147.Thoseshowingactivityareevaluatedquantitatively.Testandcontrolswatchesareinoculatedwiththetestorganisms.Afterincubation,thebacteriaareelutedfromtheswatchesbyshakinginknownamountsofneutralizingsolution.Thenumberofbacteriapresentinthisliquidisdetermined,andthepercentagereductionbythetreatedspecimeniscalculated.
3.Terminology
3.1activity,n.—ofanantibacterialagent,ameasureofeffectivenessoftheagent.
3.2antibacterialagent,n.一intextiles,anychemicalwhichkillsbacteria(bactericide)orinterfereswiththemultiplication,growthoractivityofbacteria(bacteriostat).
4.SafetyPrecautions
NOTE:
Thesesafetyprecautionsareforinformationpurposesonly.Theprecautionsareancillarytothetestingproceduresandarenotintendedtobeallinclusive.Itistheuser'sresponsibilitytousesafeandpropertechniquesinhandlingmaterialsinthistestmethod.ManufacturersMUSTbeconsultedforspecificdetailssuchasmaterialsafetydatasheetsandothermanufacturer'srecommendations.AllOSHAstandardsandrulesmustalsobeconsultedandfollowed.
4.1Boththequalitativeandquantitativetestsshouldbecarriedoutbypersonswithtrainingandexperienceintheuseofbacteriologicaltechniques.TheU.S.DepartmentofHealthandHumanServicespublication,BiosafetyinMicrobiologicalandBiomedicalLaboratories,shouldbeconsulted(see13.1).
4.2CAUTION:
Someofthebacteriausedinthistestarecapableofinfectinghumansandproducingdisease.Therefore,everynecessaryandreasonableprecautionmustbetakentoeliminatethisrisktothelaboratorypersonnelandtopersonnelintheassociatedenvironment.Wearprotectiveclothingandrespiratoryprotectionthatpreventspenetrationbythebacteria.
4.3Goodlaboratorypracticesshouldbefollowed.Wearsafetyglassesinalllaboratoryareas.
4.4Allchemicalsshouldbehandledwithcare.
4.5Aneyewash/safetyshowershouldbelocatednearbyforemergencyuse.
4.6Sterilizeallcontaminatedsamplesandtestmaterialspriortodisposal.
4.7Exposuretochemicalsusedinthisproceduremustbecontrolledatorbelowlevelssetbygovernmentauthorities(e.g.,OccupationalSafetyandHealthAdministration's[OSHA]permissibleexposurelimits[PEL]asfoundin29CFR1910.1000ofJanuary1,1989).Inaddition,theAmericanConferenceofGovernmentalIndustrialHygienists(ACGIH)ThresholdLimitValues(TLVs)comprisedoftimeweightedaverages(TUATWA),shorttermexposurelimits(TLV-STEL)andceilinglimits(TLV-C)arerecommendedasageneralguideforaircontaminantexposurewhichshouldbemet(see13.2).
5.Limitations
5.1Foraqualitative,relativelyquickandeasilyexecutedmethodtodetermineresidualantibacterialactivityoftextilematerials,refertoAATCCMethod147,AntibacterialActivityAssessmentofTextileMaterials:
ParallelStreakMethod.
6.TestOrganisms
6.1Testbacteria.
6.1.1Staphylococcusaureus,AmericanTypeCultureCollectionNo.6538.Grampositiveorganism(see13.10).
6.1.2Klebsiellapneumoniae,AmericanTypeCultureCollectionNo.4352.Gramnegativeorganism(see13.10).
6.1.3Othersuitablespeciescanalsobeused.
7.CultureMedium
7.1Suitablebroth/agarmediaareNutrient,TrypticaseSoyandBrain-HeartInfusion.
NutrientBroth:
Peptone(Bacto-peptone)
(see13.3) 5g
Beefextract(see13.4) 3g
Distilledwater to1000mL
7.2Heattoaboiltodisperseingredients.AdjusttopH6.8土0.1withINsodiumhydroxide(NaOH)solution.(Thisisnotnecessaryifprepared,dehydratedmediumisused.)
7.3Dispensein10mLamountsinconventionalbacteriologicalculturetubes(i.e.,125x17mm).Plugandsterilizeat103kPa(15psi)for15min.
7.4Nutrientagar.Ad
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