GelRunningBuffer汇总Word格式文档下载.docx

GelRunningBuffer汇总Word格式文档下载.docx

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Arginine（freebase

20mM

Lysine（freebase）

BP-211

250ml

$35

$65

IEFCathodebuffer（10X）pH3-7

BP-214

MES-SDSrunningbuffer（20X）,pH7.3 

MES

50mM

Trisbase

SDS

0.1%

EDTA

1mM 

BP-177

$42

$80

MOPS-SDSrunningbuffer（20X）,pH7.7

MOPS

Trisbase

BP-178

Tris-Acetate-SDSrunningbuffer（10X）,pH8.24

Tricine

3.5mM

BP-179

$30

$55

Tris-Glycine-SDSrunningbuffer（10X）,pH8.3

Tris-base 

25mM

Glycine

192mM

0.1%

BP-150

2000ml

4000ml

$21

$37

$70

Tris-Glycine-nativerunningbuffer（10X）,pH8.5

Tris-base

BP-160

Tris-Tricine-SDSrunningbuffer（10X）,pH8.3,（Cathodebuffer）

100mM

100mM

SDS 

BP-170

Tris-Tricine-SDSrunningbuffer（10X）,pH8.9,（Anodebuffer）

Tris-HCl 

200mM

BP-172

Tris-Tricine-SDSbuffer（10X）kit

ThekitcontainsoneeachofBP-170andBP-172

BP-175

$45

$85

TBErunningbuffer（10X）,pH8.3

89mM

Boricacid

EDTA 

2mM

BP-180

ZymogramRenaturationbuffer（10X）

TritonX1002.7%（w/v）inDIwater

BP-217

ZymogramDevelopingbuffer（10X）

Tris-HCl（pH,7.5）

NaCl 

Cacl2

5mM

Brij35

0.02%

BP-218

BlotTransferBuffer

Transferofproteinsfromthegelontothemembraneisachievedinthreeways:

simplediffusion,vacuum-assistedsolventflow,andelectrophoreticelution.Electrophoreticelutionisthemethodofchoicebecauseofitsease,speedandcompletenessoftransfer.Electrophoreticelutioncanbeachievedeitherbywetorbysemi-drytransfer.

TransferorElectroblottingbuffer（10X）,pH8.3

Justaddmethanolaccordingtoyourprotocol

BP-190

Tris-Capsbuffer（10X）

Anodebuffer,pH9.6 

Cathodebuffer,pH9.5

Tris

60mM

Caps 

40mM

Methanol（add）

15%

BP-192

$45LuminolandOxidizingSolutionsforwesternblotting（HRPBased）JustmixLuminolandOxidizingsolution,incubatethemembranefor1min.andexposethex-rayfilmfordifferentperiodsoftime.WB-100250mleach500mleach$50$85

LuminolandOxidizingSolutionsforwesternblotting（HRPBased）

JustmixLuminolandOxidizingsolution,incubatethemembranefor1min.andexposethex-rayfilmfordifferentperiodsoftime.

WB-100

250mleach

500mleach

$50

BlotBlockingBuffer

Beforetheblotcanbeprocessedforantigendetection,itisveryimportanttoblockthenon-specificbindingsitefortheantibodies.BBPprovidesfollowingreagentsthatareusedtoblockthenon-specificsitesonthemembrane.

BSA-blockingbufferinPBS

3%BSAinPBS

IBB-100

200ml

500ml

BSA-blockingbufferinTBS

3%BSAinTBS

IBB-110

200ml

Casein-blockingbufferinPBS

3%CaseininPBS

IBB-120

Casein-blockingbufferinPBS（withazide）

3%Caseinand0.02%SodiumazideinPBS

IBB-121

Casein-blockingbufferinTBS

3%CaseininTBS

IBB-130

Casein-blockingbufferinTBS（withazide）

3%Caseinand0.02%SodiumazideinTBS

IBB-131

Gelatin-blockingbufferinPBS

1%GelatininPBS

IBB-134

Gelatin-blockingbufferinPBS（withazide）

1%Gelatinand0.02%SodiumazideinPBS

IBB-135

Gelatin-blockingbufferinTBS

1%GelatininTBS

IBB-136

Gelatin-blockingbufferinTBS（withazide）

1%Gelatinand0.02%SodiumazideinTBS

IBB-137

BlottoinPBS

5%nonfatdrymilk,0.02%SodiumazideinPBS

IBB-140

$40

BlottoinTBS

5%nonfatdrymilk,0.02%SodiumazideinTBS

IBB-150

Blotto/TweeninTBS

5%nonfatdrymilk,0.1%Tween-20,0.02%SodiumazideinTBS

IBB-160

PBS/Tween-20（10X）

0.1%Tween-20inPBS

IBB-170

0.05%Tween-20inPBS

IBB-171

TBS/Tween-20（10X）

0.1%Tween-20inTBS

IBB-180

0.05%Tween-20inTBS

IBB-181

Gelatin10%

IBB-184

100ml

$60

Gelatin/TweeninPBS

1%Gelatin,0.05%Tween-20,0.02%SodiumazideinPBS

IBB-185

Gelatin/TweeninTBS

1%Gelatin,0.05%Tween-20,0.02%SodiumazideinTBS

IBB-186

HorseSerum

10%Horseserum,0.02%sodiumazideinPBS

IBB-190

BlotWashingBuffer

PleaseNote:

Phosphate-bufferedsaline 

（PBS,10X,pH7.4）

Phosphatebuffer

10mM

KCL

2.7mM

137mM

BM-220

PBS-Tween,（10X,pH7.4）

150mM

Tween-20 

0.05%

BM-221

（PBS,10X,pH7.4）

Forwesternblotwashing

SodiumPhosphatebuffer

NaCl

BM-222

Tris-bufferedsaline 

（TBS,10X）

BM-300

TBS-Tween-20,（10X.pH7.4）

（TBS,10X,pH7.4）

Tris 

BM-301

（10X,highsalt,pH7.4）

500mM

BM-301A

（TBS,10X,pH8.0）

BM-301B

BlotStrippingBuffer

Strippingbuffer,（4X）,pH6.8

250mM

8%

Justaddmercaptoethnol

BP-96

Strippingbuffer2,（4X）,pH2.5

0.2M

BP-97

MOLECULARBIOLOGY:

WORKINGWITHPROTEINS

PROTEINDETECTION:

GELELECTROPHORESIS

RUNNINGNOVEXNuPageGELS

RunningNovexNuPageGels

Title|Overview|Procedure|Solutions|BioChemicals|Hints|PrintableVersion

Contributor:

TheLaboratoryofSteveHahnattheFredHutchinsonCancerResearchCenter

Overview

ThesearesimplifiedinstructionsforuseofNovexprecastNuPagegels.TheXCEllII™minicellapparatusisrequiredforthisprotocol.FormoreinformationonNovexgelsandproductsseetheirwebpage

Procedure

A.PreparationforSDS-PAGE

1.SelectthedesiredRunningBuffer,eitherMOPS/SDSRu