胶体金试纸条技术.ppt
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- 上传时间:2022-10-24
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胶体金试纸条技术.ppt
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Thecolloidalgoldstriptestingtechnology胶体金试纸胶体金试纸条检测技术条检测技术IntroductionThecolloidalgoldstripdiagnosisisdevelopedbycolloidalgoldimmunechromatographytechnology.Thetechnologywasestablishedonthebasisoftheimmuneinfiltrationtechnologyintheearly90s.Itisasimpleandrapidimmunologicaldetectiontechnology.Thefirsttouseittodetecthumanchorionicgonadotropin(HCG)andhepatitisBvirussurfaceantigen(HBsAg),andsoon.ColloidalgodItisgoldchlorideacid(HAuCl4)hydrosol,goldchlorideacidcanaggregatintoacertainsizeofgoldparticlesundertheactionofreducingagentandbecomeastablecolloidalstatebyelectrostaticinteractions.Thequalityofthecolloidalgoldstandard:
Goodqualitycolloidalgold:
thesolutionisred,colloidalgoldparticleisspherical,uniformsize,noedgesandcorners.Poorqualitycolloidalgold:
thesolutionispurple,sizeisdiffer,avarietyofshapes.Theprincipleofcolloidalgoldlabeling:
colloidalgoldisnegativelychargedinalkalineconditions.Therefore,itcanformasolidcombinationwithproteinmoleculespositivelychargedgroupsbyelectrostaticattraction.Immunogoldlabelingtechnique:
itisanewtypeofimmunemarkerstechnology.Asatracermarker,colloidalgoldisappliedtotheantigen-antibodyreactionTheprincipleofthecolloidalgoldstripdiagnosistechnologyUsingthenitrocellulosemembraneasthecarrierandmakinguseofthecapillaryeffectofmicroporousmembrane,weaddliquidononesideofthemembrane,thentheliquidmovetotheotherendslowly.Afterthecombinationofantigen-antibody,wecantakeadvantageofthecolloidalgoldcolorreactiontodetectantigenorantibody.Itisthemostcommonlymethodtodetectantigenbyusingdoubleclampmethodholdingwire(T)Thelineofcontrolline(C)Thelineofcontrolline(C)ColloidalgoldpadabsorbentpaperSamplepadNCmembraneTomographicdirectionPVCbaseplateStripstructuremeasureWhenmeasuring,wemakethebottomoftestpaperstripimmerseintheliquidsamples,thenthebottomabsorbsliquidandmovetowardstheupperend.whentheliquidflowthroughtheC,theimmunegoldcomplexdissolveonthestrip.Ifthereareatestspecificantigeninthespecimen,itcanbecombinedwithimmuneantibodiesinthegoldcomplexes.whentheantigen-antibodycomplexflowstothetestarea,itcanbecapturedbysolidphaseantibodyandshowreactionredlineonthemembrane.Whenexcessimmunegoldcompoundsflowtothereferenceareaandcombinewithsolid-phaseanti-mouseIgG(monoclonalantibodiesintheimmunegoldcompoundsismouseIgG),thenitwillshowtheredqualitycontrolline.Negativesamplesisnoresponseline,andonlyshowqualitycontrolline.
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- 胶体 试纸 技术