常用载体及通用测序引物文档格式.docx
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- 上传时间:2022-11-17
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常用载体及通用测序引物文档格式.docx
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4752
pAAV-MCS
F(β-globin)(1192bp)
polyA)(1521bp)
4650
pAc5.1/V5-HisA
Ac5-Profor(2525bp)
BGH(2768bp)/EBVrev(2844bp)
5375
pAc5.1/V5-HisB
BGH(2772bp)/EBVrev(2848bp)
5379
pAc5.1/V5-HisC
BGH(2764bp)/EBVrev(2840bp)
5371
pAcGP67_A
PH-Profor(4016bp)
pAcGP67rv(4329bp)
9761
pAcGP67_B
pAcGP67_C
pACT
T7(1061bp)
T3(1420bp)
5566
pACT2
5'
AD(同GAL4AD)(5134bp)
3'
AD(4939bp)/pGAL4-Adrv(4964bp)(pGAL4-Adrv离MCS少于20bp,优先用3'
AD)
8117
PACYCDuet-1(MCSⅠ)
DuetUP1(3949bp)
DuetDOWN1(209bp)
Chl+
4008
PACYCDuet-1(MCSⅡ)
DuetUP2(189bp)
T7ter(468bp)
pAdTrack
pEGFP-N-3’(同EGFP-Nrev)(1294bp)
CMV-F(1433bp)
Amp+/Kan+
8111
pAdTrack-CMV
pEGFP-N-3’(同EGFP-Nrev)(1272bp)
EBVrev(1982bp)
Kan+
9010
PAO815
AOX
7700
pAS2-1
BD(同GAL4BD)(5894bp)
BD(6112bp)/pGAL4-BDrv(6046bp)(优先用3'
BD)
8392
pB42AD
pB42ADF(787bp)
pB42ADR(924bp)
6449
PBABE-IRES-Egfp
pLXSN-F(同pLXSNfw)(1027bp)/PBABE5'
(1063bp)/pBMN5'
(1416bp)
无反向引物
5944
pBabe-puro
pBabe5'
(1318bp)
pBabe3'
(1429bp)
5169
pBACPAK8
BAC1(1181bp)/PH-Profor(1174bp)/BaculoVfor(1211bp)
BAC2(1374bp)/BaculoVrev(1375bp)
5538
pBAD/HisA
pBADfw(211bp)/Xpressfor(375bp)(优先用pBADfw)
pBADrv(514bp)
4102
pBBR1MCS
M13F(3161bp)/T7(3188bp)/M13-47(3135bp)
M13R(3390bp)/T3(3352bp)/RV-M(3409bp)
4707
pBI121
35S(5735bp)
无
14758
pBK-CMV
T7(975bp)/M13F(951bp)
T3(1159bp)/M13R(1196bp)
4518
pBluescriptIIKS(-)
M13F(599bp)/T7(626bp)
T3(792bp)/M13R(829bp)
2961
pBluescriptIIKS(+)
pBluescriptIISK(-)
pBlueScriptIISK(+)
pBluescriptKS(-)
M13F(602bp)/T7(626bp)
T3(790bp)/M13R(828bp)
2958
pBluescriptKS(+)
pBluescriptSK(-)
pBluescriptSK(+)
pBSN-T
T7
T3
pBudCE4.1(MCSI)
CMV-Profor(552)/T7(638)
c-mycrev(742bp)/EBVrev(842bp)
4595
pBudCE4.2(MCSⅡ)
PEF-F(2999)
BGH(3235)
pBV220
PBV220F(3592bp)
PBV220R(93bp)
3667
pCAMBIA1300
P1(11472bp)
P2(2728bp)
11566
pCAMBIA1301
P1(10935bp)
P2(11149bp)
11849
pCAMBIA1304
P1(11454bp)
P2(11668bp)
12361
pCAMBIA2300
M13F(8418bp)/M13R(-48)(8313bp)
M13F(-47)(8461bp)/M13R(8349bp)
8742
pCANTAB_5E
pCANTAB5-S1(2265bp)/pCANTAB5-R1(2222bp)
pCANTAB5-S6(2471bp)/pCANTAB5-R2(2504bp)
4517
pCAT3-enhancer
RVP3(4215bp)
4273
pCDFDuet
T7(214bp)
T7ter(467bp)
3781
pCDH-CMV-MCS-EF1-Puro
CMV-F(2164bp)/PCEP-F(2208bp)/pLNCX-F(2210bp)
7384
pcDNA1.1
CMV-F(2076bp)/T7(2170bp)
4000
pcDNA2.1
M13F/T7
M13R/SP6
3013
pcDNA3.0
pcDNA3fw(841bp)/CMV-Profor(777bp)/T7(863bp)
SP6(1016bp)/BGH(1053bp)
5446
pcDNA3.1(-)
T7(863bp)/CMV-Profor(777bp)/pcDNA3fw(841bp)
BGH(1039bp)
5428
pcDNA3.1(+)
pcDNA3.1/HisA
T7(863bp)/CMV-Profor(777bp)
BGH(1122bp)
5514
pcDNA3.1/HisB
pcDNA3.1/HisC
pcDNA3.1/myc-HisA
BGH(1099bp)/c-mycrev(1020bp)
5494
pcDNA3.1/myc-HisB
pcDNA3.1/myc-HisC
pcDNA3.1/V5-HisA
BGH(1108bp)/V5rev(1035bp)
5502
pcDNA3.1/V5-HisB
pcDNA3.1/V5-HisC
pcDNA3.1/Zeo(-)
T7(863bp)/CMV-F(769bp)
BGH(1038bp)
5014
pcDNA3.1/Zeo(+)
5015
pcDNA3.3/TOPOTA
CMV-F(584bp)/PCEP-F(628bp)/pLNCX-F(630bp)
5407
pcDNA4/HisMaxA
Xpressfor(1119bp)
BGH(1282bp)
5258
pcDNA4/HisMaxB
pcDNA4/HisMaxC
pcDNA4/HisMax-TOPO
BGH(1302bp)
5274
pcDNA4/HisMax-TOPO/lacZ
BGH(4482bp)
8354
pcDNA4/myc-HisA
CMV-Profor(777bp)/T7(863bp)
BGH(1103bp)/c-mycrev(1024bp)
5079
pcDNA4/myc-HisB
pcDNA4/myc-HisC
CMV-Profor(777
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- 常用 载体 通用 引物