完整版Epigenetics glossary表观遗传名词解释文档格式.docx
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完整版Epigenetics glossary表观遗传名词解释文档格式.docx
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Definition
3C(ChromosomeConformationCapture)
Atechniqueusedtoquantifyspecificchromatininteractionsofinterest
invivo。
Cross-linkedrestrictiondigestedchromatinfragmentsarecircularizedtodetectinteractingDNAregionsbyPCR。
4C(CircularChromosomeConformationCapture)
A3CderivativetechniqueusedtodetectunknownDNAregionsthatinteractwitharegionofinterest.UnknownDNAinteractersareamplifiedbyprimersbindingintheknownregionwhicharethendetectedbymicroarray.
5C(ChromosomeConformationCaptureCarbonCopy)
A3CderivativetechniqueusedforindepthdetectionofDNAinteracterswithaDNAregionofinterest.5CprimersannealacrossligationjunctionstoamplytheunknowninteractingDNAlibrarywhichisthencharacterizedwithmicroarrayornext—generationsequencing。
5-Carboxylcytosine(5—caC)
Thefinaloxidisedderivativeof5—methylcytosineduringitsactivedemethylationbytheTETenzymes。
Itmayserveyetunknownbiologicalfunctionsasitsownepigeneticmark。
5-Formylcytosine(5-fc)
thepenultimateoxidisedderivativeof5—methylcytosineduringitsactivedemethylationbytheTETenzymes。
Itisenrichedatpoisedenhancersandotherregulatoryregions。
5-hydroxymethylcytosine(5hmC)
Thefirstoxidizedderivativeof5—methylcytosineduringitsactivedemethylationbytheTETenzymes.Itisenrichedinthemammalianbrainandgermcellsandmayactasitsownepigeneticmark.
Acetylation
Thecovalentadditionofanacetylgrouptoamacromolecule,mostcommonlyproteinlysineandarginineresidues。
Whenpresentonhistonesitneutralizestheirpositivechargeallowingnucleosomedecompationandenhancedgeneexpression。
Biotinylation
ThecovalentlinkageofabiotinmoleculetoamacromoleculesuchasDNAorproteintoserveasatag.Biotinisboundrapidlyandstronglybyavidinandstreptavidinproteinscreatingconjugatescoupledtofluorophorestodetectthetaggedmacromoleculetarget.
Bisulfiteconversion
TreatmentofDNAwithsodiumbisulfiteconvertingunmethylatedcytosinetouracilwhileleaving5—methylcytosine(and5-hydroxymethylcytosine)intact。
Bisulfitesequencing
AmplificationofbisulfiteconvertedDNAwithPCRfollowedbyDNAsequencingtodifferentiateunmodifiedcytosinefrom5-methylcytosine。
Itcanbeappliedtospecificregionsortheentiregenome。
BiTS-4Cseq(BatchisolateTissueSpecific4C—seq)
Amethodtodetectchromatininteractionsatalocusofinterestinaspecificcelltype,ofteninamulticellularembryo。
Aftercrosslinkingnucleiaresortedusingflowcytometrythensubjectedtotheremainderofthe4Cprotocolinparalleltothewholeembryoforcomparison。
CaptureC
Ahighthroughput,highresolution3Ctechniqueinwhicholigonucleotidecapturetechnology(OCT)isusedafterstandard3Ctoexamineinteractionsoccurringathundredsofregionsofinterestsimultaneously.
Centromere
AspecificDNAregionwhereeachpairofsisterchromatidsareconnectedduringmitosis。
Chromatininsituproximity(ChrISP)
Atechniqueforsingle—cellanalysisofproximitiesbetweentwochromatinfibersin3—Dathighresolution.Itissimilarto
insitu
proximityligationassay(ISPLA)exceptproximitiesarevisualizedbyafluorescentconnectoroligonucleotideformingacircularDNA.
ChIPonChIP
Chromatinimmunoprecipitation(ChIP)usinganantibodyagainstaproteinorhistonemodificationofinterestcoupledtomicroarraytodetectenrichedDNAregions.
Citrullination
Alsoknownasdeamination,isthepost-translationalmodificationoftheaminoacidargininetocitrullineinaprotein,reducingitspositivecharge。
CombinedBisulfiteRestrictionAnalysis(COBRA)
AtechniquetoquantifyDNAmethylationataspecificlocusfromaverysmallamountofDNAbybisulfitePCRfollowedbyrestrictiondigestionatconvertedsites.
Constitutiveheterochromatin
RegionsofDNA—particularlytelomeres,centromeres,andotherrepetitivesequences—thatarecharacterizedbyhighlycompactedtranscriptionallyinactivechromatinthatgenerallycannotbecomeactive。
CRISPR
AgenomeeditingsystemutilizingtheCRISPR/Cas9endonucleasewhichistargetedtoaDNAregionofinterestbyaspecificguideRNAtoinduceDNAdoublestrandbreaks。
Cross-linkingligationandsequencingofhybrids(CLASH)
AUVcross-linking,ligation,andsequencing-basedtechniquefortheanalysisofRNA-RNAinteractionsoccurringintheproximityofanRNAbindingproteinofinterest。
Denovomethylation
ThemethylationofcytosinesinCpGsitesthatareunmethylatedonbothDNAstrandsbydenovoDNMTs。
Deamidation
Theremovalofanamidegroupfromaprotein,particularlyconvertingasparagineresiduestoamixtureofisoaspartateandaspartate.Itisinvolve
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