蛋白质相关实验技术操作指南Word格式.doc
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蛋白质相关实验技术操作指南Word格式.doc
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ThePreussLab,TheDivisionofBiologicalSciences,TheUniversityofChicago
WesternBlotofTBPfromTBP-GSTbacteria
StevenFinkbeiner,DepartmentsofNeurologyandPhysiology,UCSF
WesternBlot/Anti-P-CREB
Westernblottingprotocolfor1C2,3B5H10,and4C8Antibodies
Protocolforanti-HAantibodyWesternBlotting
ProbingandStrippingofWesternBlot
HahnLab,TheFredHutchinsonCancerResearchCenterandHowardHughesMedicalInstitute
WesternBlottingProtocol
MirmiraLaboratoryattheUniversityofVirginia
WesternBlottingandImmunostaining
ChazinLab,CenterforStructuralBiology,VanderbiltUniversity
WesternBlots
LabofDr.MarkBartonFrank,OklahomaMedicalResearchFoundation
WesternBlotting-Antibodies
TheMinionLab,CollegeofVeterinaryMedicineatIowaStateUniversity
WesternBlotting
Cepko/TabinLab,HarvardUniversity
CarbonateSolutionForWesternBlot
Dr.DEKoshland,CarnegieInstitutionofWashington
WesternBlotwithBSA
METHODforWesternBlots
MichaelKoelle'
sLab,DepartmentofMolecularBiophysicsandBiochemistry
YaleUniversity
2、蛋白分离/提取/浓缩
LysisBuffers
YeastProteinIsolation"
Yaffe-Schatz"
AmbergLab,UpstateMedicalUniversity
GuanidineHydrochloridePurificationofProteinsFromSDSPAGE
GottschlingLab,TheFredHutchinsonCancerResearchCenterandHowardHughesMedicalInstitute
YeastproteinprepforSDSPAGEandwestern(rapid)
YeastproteinprepforSDSPAGEandwestern(glassbead)
PurificationofTFIIICfromyeast
PurificationofTFIIAfromE.coli
TCAproteinprecipitation
PurificationofMLCK(MyosinLightChainKinase)fromRabbitSkeletalMuscle肌球蛋白轻链激酶
StullLab,UniversityofTexas,SouthwesternMedicalCenter
PurificationofCalmodulinfromBovineBrain
StullLab,UniversityofTexas,SouthwesternMedicalCenter
MyosinLightChainPreparationfromSkeletalandCardiacMuscle
PurifyingProteinfromInclusionBodies
ChazinLab,CenterforStructuralBiology,VanderbiltUniversity
ProtocolforProteinExtractionfromPlant
TheUniversityofNebraska-LincolnProteinCoreFacility(PCF)
TaqPolymerasePurification
YeastProteinPrep(NoBoil)
YeastProteinPrep(Boil)
GSTFusionProteinPrep
VesicleTrafficking,StanfordUniversity
3、蛋白质检测分析:
AMIDOBLACKSTAIN
HancockLaboratoryMethods,DepartmentofMicrobiologyandImmunology,
UniversityofBritishColumbia,BritishColumbia,Canada
JamesHardwick'
sangiotensinassayprotocol
SeftonLab,SalkInstitute
Phosphoaminoacidanalysis:
MarkKamps'
smethod
SeftonLab,SalkInstitute
ULTIMATEHIS-UBASSAYFOR
theTanseyLabatColdSpringHarborLaboratory
ULTIMATEHIS-UBASSAYFORYEAST
ProtocolforBeta-HexosaminidaseRBL-2H3SecretionAssay
meyerlab,StanfordUniversity
MeasurementofMyosinRegulatoryLightChain(RLC)Phosphorylation
BicinchoninicAcid(BCA)ProteinAssay
DavidR.Caprette,RiceUniversity
BiuretProteinAssay
Bradfordproteinassay
Hartree-LowryandModifiedLowryProteinAssays
ColorimetricAssays
Kdmeasurement,fitting,calculation,andsimulation
Chazi
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