常用pGEX载体图谱Word文档格式.docx

常用pGEX载体图谱Word文档格式.docx

《常用pGEX载体图谱Word文档格式.docx》由会员分享，可在线阅读，更多相关《常用pGEX载体图谱Word文档格式.docx（30页珍藏版）》请在冰豆网上搜索。

Amyctagcanbeusedinmanydifferentassaysthatrequirerecognitionbyan 

antibody.Ifthereisnoantibodyagainstthestudiedprotein,addingamyc-tagallowsonetofollowtheproteinwithanantibodyagainsttheMycepitope.Examplesarecellularlocalizationstudiesbyimmunofluorescenceordetectionby 

Westernblotting.

Thepeptidesequenceofthemyc-tagis（in1-and3-lettercodes,respectively）:

N-EQKLISEEDL-C,N-Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu-C,whereNstandsfor 

Amino-terminus 

andCstandsfor 

Carboxyterminus.Thetagisapproximately1202 

Daltons 

inatomicmassandhas10aminoacids.

Itcanbefusedtothe 

C-terminus 

andthe 

N-terminus 

ofaprotein.Itisadvisablenottofusethetagdirectlybehindthesignalpeptideofasecretoryprotein,sinceitcaninterferewithtranslocationintothe 

secretorypathway.

Amonoclonalantibodyagainstthemyc 

epitope,named9E10,isavailablefromthenon-commercial 

DevelopmentalStudiesHybridomaBank

pGEX4T1载体基本信息

出品公司:

GE

别名:

pGEX-4T-1,pGEX4T1,pGEX4T1

质粒类型:

大肠杆菌蛋白表达载体

表达水平:

高拷贝

启动子:

Tac

克隆方法:

多克隆位点，限制性内切酶

载体大小:

4969bp

5'

测序引物及序列:

pGEX5'

:

GGGCTGGCAAGCCACGTTTGGTG

3'

pGEX3'

CCGGGAGCTGCATGTGTCAGAGG

载体标签:

N-GST

载体抗性:

Ampicillin氨苄

备注:

复制子是pMB1

产品目录号:

27-4580-01

稳定性:

瞬时表达Transient

组成型:

诱导表达

病毒/非病毒:

非病毒

pGEX4T1载体质粒图谱和多克隆位点信息

原核生物DNA复制起始点，是DNA链上独特的具有起始DNA复制功能的碱基序列。

大肠杆菌的复制起点包括OriC和OriH

Thrombin凝血酶

pGEX4T1载体简介

pGEX4T1载体序列

LOCUSpGEX-4T-14969bpDNAcircularSYN

DEFINITIONpGEX-4T-1

ACCESSION

KEYWORDS

SOURCE

ORGANISMothersequences;

artificialsequences;

vectors.

COMMENT 

ThisfileiscreatedbyVectorNTI

VNTAUTHORNAME||

FEATURESLocation/Qualifiers

source1..4969

/organism="

pGEX-4T-1"

/mol_type="

otherDNA"

promoter184..212

/label="

tac_promoter"

misc_feature224..246

M13_pUC_rev_primer"

gene258..977

GST（variant）"

/gene="

CDS258..977

ORFframe3"

misc_featurecomplement（1019..1041）

pGEX_3_primer"

promoter1307..1335

AmpR_promoter"

gene1377..2237

Ampicillin"

CDS1377..2237

rep_origin2392..3011

pBR322_origin"

misc_feature3309..4400

lacI"

CDS3441..4400

promoter4449..4478

lac_promoter"

misc_feature4492..4514

promoter4513..4531

M13_reverse_primer"

CDS4523..81

ORFframe2"

misc_feature4540..4695

lacZ_a"

promotercomplement（4543..4559）

M13_forward20_primer"

misc_featurecomplement（4552..4574）

M13_pUC_fwd_primer"

pGEX4T2载体基本信息

pGEX-4T-2,pGEX4T2,pGEX4T2

4970bp

27-4581-01

pGEX4T2载体质粒图谱和多克隆位点信息

pGEX4T2载体简介

pGEX4T2载体序列

LOCUSpGEX-4T-24970bpDNAcircularSYN

DEFINITIONpGEX-4T-2

source1..4970

pGEX-4T-2"

gene258..978

CDS258..974

misc_featurecomplement（1020..1042）

promoter1308..1336

gene1378..2238

CDS1378..2238

ORFframe1"

rep_origin2393..3012

misc_feature3310..4401

CDS3442..4401

promoter4450..4479

misc_feature4493..4515

promoter4514..4532

CDS4524..81

misc_feature4541..4696