常用荧光染料的激发及发射波长.docx
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常用荧光染料的激发及发射波长.docx
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常用荧光染料的激发及发射波长
荧光素名称
激发波长(nm)
发射波长(nm)
备注
1,5IAEDANS
336
490
1,8-ANS
372
480
4-Methylumbelliferone
385
502
5-carboxy-2,7-dichlorofluorescein
504
529
5-Carboxyfluorescein(5-FAM)
492
518
5-Carboxynapthofluorescein(pH10)
512/598
563/668
RatioDye,pH
5-Carboxytetramethylrhodamine(5-TAMRA)
542
568
5-FAM(5-Carboxyfluorescein)
492
518
5-HAT(HydroxyTryptamine)
370-415
520-540
5-HydroxyTryptamine(HAT)
370-415
520-540
5-ROX(carboxy-X-rhodamine)
578
567
604
591
5-TAMRA(5-Carboxytetramethylrhodamine)
548
542
552
568
6-Carboxyrhodamine6G
518
543
6-CR6G
518
543
6-JOE
520
548
7-Amino-4-methylcoumarin
351
430
7-AminoactinomycinD(7-AAD)
546
647
7-Hydroxy-4-methylcoumarin
360
449,455
9-Amino-6-chloro-2-methoxyacridine
412,430
471,474
ABQ
344
445
AcidFuchsin
540
630
ACMA(9-Amino-6-chloro-2-methoxyacridine)
412,430
471,474
AcridineOrange+DNA
502
526
AcridineOrange+RNA
460
650
AcridineOrange,bothDNA&RNA
440-480
520-650
AcridineRed
455-600
560-680
AcridineYellow
470
550
Acriflavin
436
520
AcriflavinFeulgenSITSA
355-425
460
Aequorin(Photoprotein)
466
Photoprotein
AFPs-AutoFluorescentProtein-(QuantumBiotechnologies)seesgGFP,sgBFP
AlexaFluor350™
346
342
442
441
AlexaFluor430™
431
540
AlexaFluor488™
495,492
519,520
AlexaFluor532™
531,532
553,554
AlexaFluor546™
556,557
572,573
AlexaFluor568™
577,578
603
AlexaFluor594™
590,594
617,618
AlexaFluor633™
632
650
AlexaFluor647™
647
666
AlexaFluor660™
668
698
AlexaFluor680™
679
702
AlizarinComplexon
530-560,580
580,624-645
AlizarinRed
530-560
580
Allophycocyanin(APC)
630,645
655,660
AMC,AMCA-S
345
445
AMCA(Aminomethylcoumarin)
345
347
425
444
AMCA-X
353
442
AminoactinomycinD
555
655
Aminocoumarin
346
350
442
445
Aminomethylcoumarin(AMCA)
345
347
425
444
AnilinBlue
600
Anthrocylstearate
360-381
446
APC(Allophycocyanin)
630,645
655,660
APC-Cy7
625-650
755
APTRA-BTC=RatioDye,Zn2+
466/380
520/530
RatioDye,Zn2+
APTS
424
505
AstrazonBrilliantRed4G
500
585
AstrazonOrangeR
470
540
AstrazonRed6B
520
595
AstrazonYellow7GLL
450
480
Atabrine
436
490
ATTO-TAG™CBQCA
465
560
ATTO-TAG™FQ
486
591
Auramine
460
550
AurophosphineG
450
580
Aurophosphine
450-490
515
BAO9(Bisaminophenyloxadiazole)
365
395
BCECF(highpH)
492,503
520,528
BCECF(lowpH)
482
520
BerberineSulphate
430
550
BetaLactamase
409
447,520
BFPblueshiftedGFP(Y66H)
BlueFluorescentProtein
381,382,383
445,447,448
blueshiftedGFP(Y66H)
BlueFluorescentProtein
BFP/GFPFRET
Bimane
398
490
Bisbenzamide
360
461
Bisbenzimide(Hoechst)
360
461
bis-BTC=RatioDye,Zn2+
455/405
529/505
RatioDye,Zn2+
BlancophorFFG
390
470
BlancophorSV
370
435
BOBO™-1
462
481
BOBO™-3
570
602
Bodipy492/515
490
515
Bodipy493/503
533
549
Bodipy500/510
509
515
Bodipy505/515
502
510
Bodipy530/550
528
547
Bodipy542/563
543
563
Bodipy558/568
558
569
Bodipy564/570
564
570
Bodipy576/589
579
590
Bodipy581/591
584
592
Bodipy630/650-X
625
642
Bodipy650/665-X
647
665
Bodipy665/676
605
676
BodipyFl
504,505
511,513
BodipyFLATP
505
514
BodipyFl-Ceramide
505
511
BodipyR6GSE
528
547
BodipyTMR
542
574
BodipyTMR-Xconjugate
544
573
BodipyTMR-X,SE
544
570
BodipyTR
589
617
BodipyTRATP
591
620
BodipyTR-XSE
588
616
BO-PRO™-1
462
481
BO-PRO™-3
544
570
BrilliantSulphoflavinFF
430
520
BTC-RatioDyeCa2+
464/401
533/529
RatioDyeCa2+
BTC-5N-atioDye,Zn2+
459/417
517/532
RatioDye,Zn2+
Calcein
494
517
CalceinBlue
373
440
CalciumCrimson™
588,589
611,615
CalciumGreen
501,506
531
CalciumGreen-1Ca2+Dye
506
531
Ca2+Dye
CalciumGreen-2Ca2+
506/503
536
Ca2+
CalciumGreen-5NCa2+
506
532
Ca2+
CalciumGreen-C18Ca2+
509
530
Ca2+
CalciumOrange
549
575
576
CalcofluorWhite
385,395,405
437,440,445
Carboxy-X-rhodamine(5-ROX)
576
601
CascadeBlue™
377
398
399
420
423
CascadeYellow
399
400
550
552
Catecholamine
410
470
CCF2(GeneBlazer)
CFDA
494
520
CFP-CyanFluorescentProtein
430,433,436,(453)
474,475,476,(501)
CyanFluorescentProtein
CFP/YFPFRET
Chlorophyll
480
650
ChromomycinA
436-460
470
ChromomycinA
445
575
CL-NERF(RatioDye,pH)
504/514
540
RatioDye,pH
CMFDA
494
520
CoelenterazineCa2+Dye,bioluminescence
(429)
465
Ca2+Dye,bioluminescence,
nativemolecule
Coelenterazinecp(Ca2+Dye,)
(430)
442
Ca2+Dye,bioluminescence
Coelenterazinef
(437)
473
Ca2+Dye,bioluminescence
Coelenterazinefcp
452
Ca2+Dye,bioluminescence
Coelenterazineh
(437)
464
Ca2+Dye,bioluminescence
Coelenterazinehcp
(433)
444
Ca2+Dye,bioluminescence
Coelenterazineip
441
Ca2+Dye,bioluminescence
Coelenterazinen
(431)
467
Ca2+Dye,bioluminescence
CoelenterazineO
460
575
CoumarinPhalloidin
387
470
C-phycocyanine
CPMMethylcoumarin
384
469
Methylcoumarin
CTC
400-450
602
CTCFormazan
Cy2™
489
506
Cy3.18
554
568
Cy3.5™
581
598
Cy3™
514
552
554
566
570
Cy5.18
649
666
Cy5.5™
675
695
Cy5™
649
666
670
Cy7™
710,743
767,805
CyanGFP
433(453)
475(501)
cyclicAMPFluorosensor(FiCRhR)
500
517
CyQuantCellProliferationAssay
480
520
CellProliferationAssay
Dabcyl
453
Dansyl
340
578
DansylAmine
337
517
DansylCadaverine
335
518
DansylChloride
372
518
DansylDHPE
336
517
Dansylfluoride
356
none
DAPI
359
461
Dapoxyl
403
580
Dapoxyl2
374
574
Dapoxyl3
373
574
DCFDA
504
529
DCFH(DichlorodihydrofluoresceinDiacetate)
505
535
DDAO
463
607
DHR(Dihydorhodamine123)
505
534
Di-4-ANEPPS
496
705
Di-8-ANEPPS(non-ratio)
488
498
605
713
DiA(4-Di-16-ASP)
456
591
DichlorodihydrofluoresceinDiacetate(DCFH)
505
535
DiD-LipophilicTracer
644
665
LipophilicTracer
DiD(DiIC18(5))
644
665
DIDS
341
415
Dihydorhodamine123(DHR)
505
535
DiI(DiIC18(3))
549,551
565
Dinitrophenol
349
DiO(DiOC18(3))
484,487
501,502
DiR
748
780
LipophilicTracer
DiR(DiIC18(7))
750
779
DM-NERF(highpH)
497/510
540
RatioDye,pH
DNP
349
Dopamine
340
490-520
DsRed
558
583
Redfluorescentprotein
DTAF
494
520
DY-630-NHS
621
660
Hemicyane
labelforproteinsandDNA
DY-635-NHS
634
664
Hemicyane
labelforproteinsandDNA
EBFP
383
447
EnhancedBlueFluorescentProtein
ECFP
436
474
EnhancedCyanFluorescentProtein
EGFP
488,498
507,516
EnhancedGreenFluorescentProtein
ELF97
345
530
Eosin
524
545
Erythrosin
529,532
554,555
ErythrosinITC
529
555
EthidiumBromide
510,523
595,605
Ethidiumhomodimer-1(EthD-1)
528
617
Euchrysin
430
540
EukoLight
Europium(III)chloride
EYFP
513,520
527,532
EnhancedYellowFluorescentProtein
FastBlue
360
440
FDA
494
520
Feulgen(Pararosaniline)
570
625
FIF(FormaldehydInducedFluorescence)
405
433
FITC
490,494
520,525
FITCAntibody
493
517
FlazoOrange
375-530
612
Fluo-3
480–506,506
520,527
Fluo-4
494
516
Fluorescein(FITC)
490,494
520,525
FluoresceinDiacetate
494
520
Fluoro-Emerald
495
524
Fluoro-Gold(Hydroxystilbamidine)
361
536
Fluor-Ruby
555
582
FluorX
494
520
FM1-43™
479
598
FM4-46
515
640
FuraRed™(highpH)
572
657
FuraRed™/Fluo-3
Fura-2,highcalcium
335
505
Excitationratiodye
Fura-2,lowcalcium
363
512
Excitationratiodye
Fura-2/BCECF
GenacrylBrilliantRedB
520
590
GenacrylBrilliantYellow10GF
430
485
GenacrylPink3G
470
583
GenacrylYellow5GF
430
475
GeneBlazer(CCF2)
GFP(S65T)
498
516
GFPredshifted(rsGFP)
498
516
GFPwildtype,non-UVexcitation(wtGFP)
475
509
GFPwildtype,UVexcitation(wtGFP)
395
509
GFPuv
385
508
GloxalicAcid
405
460
GranularBlue
355
425
Haematoporphyrin
530-560
580
Hoechst33258
345
487
Hoechst33342
347
483
Hoechst34580
392
440
HPTS
355
465
Hydroxycoumarin
325-360
386-455
Hydroxystilbamidine(FluoroGold)
361
536
Hydroxytryptamine
400
530
Indo-1,highcalcium
330
401
Emissionratiodye
Indo-1,lowcalcium
346
475
Emissionratiodye
Indodicarbocyanine(DiD)
644
665
Indotricarbocyanine(DiR)
748
780
IntrawhiteCf
360
430
JC-1
514
529
JO-JO-1
530
545
JO-PRO-1
532
544
LaserPro
795
812
Laurodan
355
460
LDS751(DNA)
543
712
LDS751(RNA)
590
607
LeucophorPAF
370
430
LeucophorSF
380
465
LeucophorWS
395
465
LissamineRhodamine
572,577
591,592
LissamineRhodamineB
577
592
LIVE/DEADKitAnimalCells
Calcein/Ethidiumhomodimer
494
528
517
617
formoredetailsrefer
to
LOLO-1
566
580
LO-PRO-1
568
581
LuciferYellow
425,428
528,536,540
LysoTrackerBlue
373
422
LysoTrackerBlue-White
466
536
LysoTrackerGreen
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- 常用 荧光 染料 激发 发射 波长
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